DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryanggeun Lee | - |
dc.contributor.author | Keunsang Yang | - |
dc.contributor.author | Jong-Bong Lee | - |
dc.date.accessioned | 2019-12-11T08:50:13Z | - |
dc.date.available | 2019-12-11T08:50:13Z | - |
dc.date.created | 2019-07-23 | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/100498 | - |
dc.description.abstract | Single-molecule techniques have been used successfully to visualize real-time enzymatic activities, revealing transient complex properties and heterogeneity of various biological events. Especially, conventional force spectroscopy including optical tweezers and magnetic tweezers has been widely used to monitor change in DNA length by enzymes with high spatiotemporal resolutions of similar to nanometers and similar to milliseconds. However, DNA metabolism results from coordination of a number of components during the processes, requiring efficient monitoring of a complex of proteins catalyzing DNA substrates. In this min-review, we will introduce a simple and multiplexed single-molecule assay to detect DNA substrates catalyzed by enzymes with high-throughput data collection. We conclude with a perspective of possible directions that enhance capability of the assay to reveal complex biological events with higher resolution. | - |
dc.language | English | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.relation.isPartOf | BMB REPORTS | - |
dc.title | Multiplexed single-molecule flow-stretching bead assay for DNA enzymology | - |
dc.type | Article | - |
dc.identifier.doi | 10.5483/BMBRep.2019.52.10.170 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BMB REPORTS, v.52, no.10, pp.589 - 594 | - |
dc.identifier.wosid | 000494337500002 | - |
dc.citation.endPage | 594 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 589 | - |
dc.citation.title | BMB REPORTS | - |
dc.citation.volume | 52 | - |
dc.contributor.affiliatedAuthor | Ryanggeun Lee | - |
dc.contributor.affiliatedAuthor | Keunsang Yang | - |
dc.contributor.affiliatedAuthor | Jong-Bong Lee | - |
dc.identifier.scopusid | 2-s2.0-85074184317 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Review | - |
dc.subject.keywordPlus | FORCE SPECTROSCOPY | - |
dc.subject.keywordPlus | OPTICAL TWEEZERS | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | REVEAL | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordAuthor | Flow-stretching bead assay | - |
dc.subject.keywordAuthor | Force spectroscopy | - |
dc.subject.keywordAuthor | Multiplexed single-molecule assay | - |
dc.subject.keywordAuthor | Single-molecule enzymology | - |
dc.subject.keywordAuthor | Single-molecule technique | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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