Structural and biophysical properties of RIG-I bound to dsRNA with G-U wobble base pairs.
SCIE
SCOPUS
- Title
- Structural and biophysical properties of RIG-I bound to dsRNA with G-U wobble base pairs.
- Authors
- Kim, Ki-Hun; Hwang, Kihyun; Kim, Jin Hong; Son, Kyung-Pyo; Jang, Yejin; Kang, Suk-Jo; Lee, Jie-Oh; Kang, Jin Young; Choi, Byong-Seok
- Date Issued
- 2020-03
- Publisher
- LANDES BIOSCIENCE
- Abstract
- Retinoic acid-inducible gene I (RIG-I) is responsible for innate immunity via the recognition of short double-stranded RNAs in the cytosol. With the clue that G-U wobble base pairs in the influenza A virus's RNA promoter region are responsible for RIG-I activation, we determined the complex structure of RIG-I Delta CARD and a short hairpin RNA with G-U wobble base pairs by X-ray crystallography. Interestingly, the overall helical backbone trace was not affected by the presence of the wobble base pairs; however, the base pair inclination and helical axis angle changed upon RIG-I binding. NMR spectroscopy revealed that RIG-I binding renders the flexible base pair of the influenza A virus's RNA promoter region between the two G-U wobble base pairs even more flexible. Binding to RNA with wobble base pairs resulted in a more flexible RIG-I complex. This flexible complex formation correlates with the entropy-favoured binding of RIG-I and RNA, which results in tighter binding affinity and RIG-I activation. This study suggests that the structure and dynamics of RIG-I are tailored to the binding of specific RNA sequences with different flexibility.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/101336
- DOI
- 10.1080/15476286.2019.1700034
- ISSN
- 1547-6286
- Article Type
- Article
- Citation
- RNA Biology, vol. 17, no. 3, page. 325 - 334, 2020-03
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- There are no files associated with this item.
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