DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SK | - |
dc.contributor.author | Choi, JH | - |
dc.contributor.author | Suh, PG | - |
dc.contributor.author | Chang, JS | - |
dc.date.accessioned | 2015-06-25T01:55:39Z | - |
dc.date.available | 2015-06-25T01:55:39Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2006-06-30 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.other | 2015-OAK-0000006067 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/10194 | - |
dc.description.abstract | Phosphoinositide-specific phospholipase C-gamma 1 (PLC-gamma 1) has two pleckstrin homology (PH) domains: an amino-terminal domain (M) and a split PH domain (PH2). Here, we show that overlay assay of bovine brain tubulin pool with glutathione-S-transferase (GST)-PLC-gamma 1 PH domain fusion proteins, followed by matrix-assisted laser-desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), identified 68-kDa neurofilament light chain (NF-L) as a binding protein of amino-terminal PH domain of PLC-gamma 1. NF-L is known as a component of neuronal intermediate filaments, which are responsible for supporting the structure of myelinated axons in neuron. PLC-gamma 1 and NF-L colocalized in the neurite in PC12 cells upon nerve growth factor stimulation. In vitro binding assay and immunoprecipitation analysis also showed a specific interaction of both proteins in differentiated PC12 cells. The phosphatidylinositol 4, 5-bisphosphate [PI(4,5)P-2] hydrolyzing activity of PLC-gamma 1 was slightly decreased in the presence of purified NF-L in vitro, suggesting that NF-L inhibits PLC-gamma 1. Our results suggest that PLC-gamma 1-associated NF-L sequesters the phospho lipid from the PH domain of PLC-gamma 1. | - |
dc.description.statementofresponsibility | open | en_US |
dc.language | English | - |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | Pleckstrin homology domain of phospholipase C-gamma 1 directly binds to 68-kDa neurofilament light chain | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | en_US |
dc.identifier.doi | 10.1038/emm.2006.32 | - |
dc.author.google | Kim, SK | en_US |
dc.author.google | Choi, JH | en_US |
dc.author.google | Chang, JS | en_US |
dc.author.google | Suh, PG | en_US |
dc.relation.volume | 38 | en_US |
dc.relation.issue | 3 | en_US |
dc.relation.startpage | 265 | en_US |
dc.relation.lastpage | 272 | en_US |
dc.contributor.id | 10052640 | en_US |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | en_US |
dc.relation.index | SCI급, SCOPUS 등재논문 | en_US |
dc.relation.sci | SCI | en_US |
dc.collections.name | Journal Papers | en_US |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.38, no.3, pp.265 - 272 | - |
dc.identifier.wosid | 000238925500010 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 272 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 265 | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 38 | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.identifier.scopusid | 2-s2.0-33745847751 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 9 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | AMYOTROPHIC-LATERAL-SCLEROSIS | - |
dc.subject.keywordPlus | SLOW AXONAL-TRANSPORT | - |
dc.subject.keywordPlus | PH DOMAIN | - |
dc.subject.keywordPlus | INTERMEDIATE-FILAMENTS | - |
dc.subject.keywordPlus | BETA-TUBULIN | - |
dc.subject.keywordPlus | MOUSE MODEL | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | C-GAMMA-1 | - |
dc.subject.keywordAuthor | neurofilament protein L | - |
dc.subject.keywordAuthor | PC12 cells | - |
dc.subject.keywordAuthor | phospholipase C gamma | - |
dc.subject.keywordAuthor | phosphatidylinositol 4,5-diphosphate | - |
dc.subject.keywordAuthor | protein interaction mapping | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.