DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sanghak Cha | - |
dc.contributor.author | Hyun Gyu Lim | - |
dc.contributor.author | Seokmu Kwon | - |
dc.contributor.author | Dong-hwan Kim | - |
dc.contributor.author | Chae Won Kang | - |
dc.contributor.author | Gyoo Yeol Jung | - |
dc.date.accessioned | 2021-06-01T02:01:13Z | - |
dc.date.available | 2021-06-01T02:01:13Z | - |
dc.date.created | 2021-03-07 | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/105147 | - |
dc.description.abstract | Carbon monoxide (CO) is a promising carbon source for producing value-added biochemicals via microbial fermentation. However, its microbial conversion has been challenging because of difficulties in genetic engineering of CO-utilizing microorganisms and, more importantly, maintaining CO consumption which is negatively affected by the toxicity of CO and accumulated byproducts. To overcome these issues, we devised mutualistic microbial consortia, co-culturing Eubacterium limosum and genetically engineered Escherichia coli for the production of 3-hydroxypropionic acid (3-HP) and itaconic acid (ITA). During the co-culture, E. limosum assimilated CO and produced acetate, a toxic by-product, while E. coli utilized acetate as a sole carbon source. We found that this mutualistic interaction dramatically stabilized and improved CO consumption of E. limosum compared to monoculture. Consequently, the improved CO consumption allowed successful production of 3-HP and ITA from CO. This study is the first demonstration of value-added biochemical production from CO using a microbial consortium. Moreover, it suggests that synthetic mutualistic microbial consortium can serve as a powerful platform for the valorization of CO. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | METABOLIC ENGINEERING | - |
dc.title | Design of mutualistic microbial consortia for stable conversion of carbon monoxide to value-added chemicals | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ymben.2021.02.001 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.64, pp.146 - 153 | - |
dc.identifier.wosid | 000631887200004 | - |
dc.citation.endPage | 153 | - |
dc.citation.startPage | 146 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 64 | - |
dc.contributor.affiliatedAuthor | Sanghak Cha | - |
dc.contributor.affiliatedAuthor | Dong-hwan Kim | - |
dc.contributor.affiliatedAuthor | Chae Won Kang | - |
dc.contributor.affiliatedAuthor | Gyoo Yeol Jung | - |
dc.identifier.scopusid | 2-s2.0-85100654719 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Microbial consortia | - |
dc.subject.keywordAuthor | Carbon monoxide | - |
dc.subject.keywordAuthor | Fermentation stability | - |
dc.subject.keywordAuthor | 3-Hydroxypropionic acid | - |
dc.subject.keywordAuthor | Itaconic acid | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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