HIGH-RESOLUTION WHOLE ORGAN IMAGING USING TWO-PHOTON TISSUE CYTOMETRY
SCIE
SCOPUS
- Title
- HIGH-RESOLUTION WHOLE ORGAN IMAGING USING TWO-PHOTON TISSUE CYTOMETRY
- Authors
- Ragan, T; Sylvan, JD; Kim, KH; Huang, H; Bahlmann, K; Lee, RT; So, PTC
- Date Issued
- 2007-01
- Publisher
- SPIE-INT SOCIETY OPTICAL ENGINEERING
- Abstract
- Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales. (c) 2007 Society of Photo-Optical Instrumentation Engineers.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/10667
- DOI
- 10.1117/1.2435626
- ISSN
- 1083-3668
- Article Type
- Article
- Citation
- JOURNAL OF BIOMEDICAL OPTICS, vol. 12, no. 1, page. 14015 - 14018, 2007-01
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