DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, WL | - |
dc.contributor.author | Chou, CK | - |
dc.contributor.author | Lin, MG | - |
dc.contributor.author | Chen, YF | - |
dc.contributor.author | Jee, SH | - |
dc.contributor.author | Tan, HY | - |
dc.contributor.author | Tsai, TH | - |
dc.contributor.author | Kim, KH | - |
dc.contributor.author | Kim, D | - |
dc.contributor.author | So, PTC | - |
dc.contributor.author | Lin, SJ | - |
dc.contributor.author | Dong, CY | - |
dc.date.accessioned | 2015-06-25T02:17:07Z | - |
dc.date.available | 2015-06-25T02:17:07Z | - |
dc.date.created | 2010-01-18 | - |
dc.date.issued | 2009-09 | - |
dc.identifier.issn | 1083-3668 | - |
dc.identifier.other | 2015-OAK-0000019786 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/10669 | - |
dc.description.abstract | Both reflected confocal and multiphoton microscopy can have clinical diagnostic applications. The successful combination of both modalities in tissue imaging enables unique image contrast to be achieved, especially if a single laser excitation wavelength is used. We apply this approach for skin and corneal imaging using the 780-nm output of a femtosecond, titanium- sapphire laser. We find that the near-IR, reflected confocal (RC) signal is useful in characterizing refractive index varying boundaries in bovine cornea and porcine skin, while the multiphoton autofluorescence (MAF) and second- harmonic generation (SHG) intensities can be used to image cytoplasm and connective tissues (collagen), respectively. In addition, quantitative analysis shows that we are able to detect MAF from greater imaging depths than with the near-IR RC signal. Furthermore, by performing RC imaging at 488, 543, and 633 nm, we find that a longer wavelength leads to better image contrast for deeper imaging of the bovine cornea and porcine skin tissue. Finally, by varying power of the 780-nm source, we find that comparable RC image quality was achieved in the 2.7 to 10.7-mW range. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3247157] | - |
dc.description.statementofresponsibility | open | en_US |
dc.language | English | - |
dc.publisher | SPIE-SOC PHOTOPTICAL INSTRUMENTATION | - |
dc.relation.isPartOf | JOURNAL OF BIOMEDICAL OPTICS | - |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | Single-wavelength reflected confocal and multiphoton microscopy for tissue imaging | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | en_US |
dc.identifier.doi | 10.1117/1.3247157 | - |
dc.author.google | Chen, WL | en_US |
dc.author.google | Chou, CK | en_US |
dc.author.google | Dong, CY | en_US |
dc.author.google | Lin, SJ | en_US |
dc.author.google | So, PTC | en_US |
dc.author.google | Kim, D | en_US |
dc.author.google | Kim, KH | en_US |
dc.author.google | Tsai, TH | en_US |
dc.author.google | Tan, HY | en_US |
dc.author.google | Jee, SH | en_US |
dc.author.google | Chen, YF | en_US |
dc.author.google | Lin, MG | en_US |
dc.relation.volume | 14 | en_US |
dc.relation.issue | 5 | en_US |
dc.relation.startpage | 54026 | en_US |
dc.relation.lastpage | 54026 | en_US |
dc.contributor.id | 10183385 | en_US |
dc.relation.journal | JOURNAL OF BIOMEDICAL OPTICS | en_US |
dc.relation.index | SCI급, SCOPUS 등재논문 | en_US |
dc.relation.sci | SCI | en_US |
dc.collections.name | Journal Papers | en_US |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOMEDICAL OPTICS, v.14, no.5, pp.54026 - 54026 | - |
dc.identifier.wosid | 000271746100033 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 54026 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 54026 | - |
dc.citation.title | JOURNAL OF BIOMEDICAL OPTICS | - |
dc.citation.volume | 14 | - |
dc.contributor.affiliatedAuthor | Kim, KH | - |
dc.identifier.scopusid | 2-s2.0-74049128998 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 12 | - |
dc.description.scptc | 13 | * |
dc.date.scptcdate | 2018-10-274 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | EXCITATION FLUORESCENCE MICROSCOPY | - |
dc.subject.keywordPlus | SCANNING LASER MICROSCOPY | - |
dc.subject.keywordPlus | BASAL-CELL CARCINOMA | - |
dc.subject.keywordPlus | 2ND-HARMONIC GENERATION | - |
dc.subject.keywordPlus | HUMAN SKIN | - |
dc.subject.keywordPlus | EX-VIVO | - |
dc.subject.keywordPlus | CORNEA | - |
dc.subject.keywordPlus | EYE | - |
dc.subject.keywordAuthor | multiphoton fluorescence | - |
dc.subject.keywordAuthor | second-harmonic generation | - |
dc.subject.keywordAuthor | reflected confocal imaging | - |
dc.subject.keywordAuthor | optical microscopy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Optics | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
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