Characterization of the Gel-Spun Tubular Scaffold for Cardiovascular Tissue Engineering

Abstract

A tubular and fibrous scaffold was fabricated from an elastic polymer, poly (L-lactideco-epsilon-caprolactone) (PLCL; Mn 193,813, Mw 538,623) 50:50 by using a novel gel spinning apparatus. To characterize the gel-spun scaffold, we investigated morphology, tensile property, tissue in-growth rate and degradation rate. From SEM images, fibrous structure in the scaffold wasn't fabricated well in the condition of 4% gel concentration. In general, the thickness level of microfibers increased as the gel concentration increased. In addition, the gel-spun scaffolds showed stronger tensile properties in the circumferential direction than the longitudinal direction. 5%, 7.5%, 10% and 12.5% scaffolds were analyzed in both directions: circumferential direction and longitudinal direction. On the other hand, the gel-spun scaffolds have been implanted in mouse to examine the degradation rate in vivo and tissue in-growth aspects, compared to extruded scaffolds. Both shows very similar degradation rates, but the aspect in tissue in-growth was different. In conclusion, gel-spun PLCL scaffolds have good characteristics as a plausible scaffold for cardiovascular tissue engineering.

A tubular and fibrous scaffold was fabricated from an elastic polymer, poly (L-lactideco-epsilon-caprolactone) (PLCL; Mn 193,813, Mw 538,623) 50:50 by using a novel gel spinning apparatus. To characterize the gel-spun scaffold, we investigated morphology, tensile property, tissue in-growth rate and degradation rate. From SEM images, fibrous structure in the scaffold wasn't fabricated well in the condition of 4% gel concentration. In general, the thickness level of microfibers increased as the gel concentration increased. In addition, the gel-spun scaffolds showed stronger tensile properties in the circumferential direction than the longitudinal direction. 5%, 7.5%, 10% and 12.5% scaffolds were analyzed in both directions: circumferential direction and longitudinal direction. On the other hand, the gel-spun scaffolds have been implanted in mouse to examine the degradation rate in vivo and tissue in-growth aspects, compared to extruded scaffolds. Both shows very similar degradation rates, but the aspect in tissue in-growth was different. In conclusion, gel-spun PLCL scaffolds have good characteristics as a plausible scaffold for cardiovascular tissue engineering.