DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim H. | - |
dc.contributor.author | Kwon K.W. | - |
dc.contributor.author | Park J. | - |
dc.contributor.author | Kang H. | - |
dc.contributor.author | Lee Y. | - |
dc.contributor.author | Sohn E.-J. | - |
dc.contributor.author | Hwang I. | - |
dc.contributor.author | Eum S.-Y. | - |
dc.contributor.author | Shin S.J. | - |
dc.date.accessioned | 2021-12-03T09:24:17Z | - |
dc.date.available | 2021-12-03T09:24:17Z | - |
dc.date.created | 2020-07-23 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.issn | 2076-393X | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/107878 | - |
dc.description.abstract | Tuberculosis (TB) is one of the deadliest infectious diseases worldwide and is caused byMycobacterium tuberculosis(Mtb). An effective vaccine to prevent TB is considered the most cost-effective measure for controlling this disease. Many different vaccine antigen (Ag) candidates, including well-known and newly identified Ags, have been evaluated in clinical and preclinical studies. In this study, we took advantage of a plant system of protein expression usingNicotiana benthamianato produce N-glycosylated antigen 85A (G-Ag85A), which is one of the most well-characterized vaccine Ag candidates in the field of TB vaccines, and compared its immunogenicity and vaccine efficacy with those of nonglycosylated Ag85A (NG-Ag85A) produced with anEscherichia colisystem. Notably, G-Ag85A induced a more robust IFN-gamma response than NG-Ag85A, which indicated that G-Ag85A is well recognized by the host immune system during Mtb infection. We subsequently compared the vaccine potential of G-Ag85A and NG-Ag85A by evaluating their immunological features and substantial protection efficacies. Interestingly, G-Ag85A yielded moderately enhanced long-term protective efficacy, as measured in terms of bacterial burden and lung inflammation. Strikingly, G-Ag85A-immunized mice showed a more balanced proportion of multifunctional Th1-biased immune responses with sustained IFN-gamma response than did NG-Ag85A-immunized mice. Collectively, plant-derived G-Ag85A could induce protective and balanced Th1 responses and confer long-term protection against a hypervirulent Mtb Beijing strain infection, which indicated that plant-produced G-Ag85A might provide an excellent example for the production of an Mtb subunit vaccine Ag and could be an effective platform for the development of anti-TB vaccines. | - |
dc.language | English | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.relation.isPartOf | Vaccines | - |
dc.title | Plant-Produced N-glycosylated Ag85A Exhibits Enhanced Vaccine Efficacy Against Mycobacterium tuberculosis HN878 Through Balanced Multifunctional Th1 T Cell Immunity | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/vaccines8020189 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Vaccines, v.8, no.2 | - |
dc.identifier.wosid | 000550922400001 | - |
dc.citation.number | 2 | - |
dc.citation.title | Vaccines | - |
dc.citation.volume | 8 | - |
dc.contributor.affiliatedAuthor | Sohn E.-J. | - |
dc.contributor.affiliatedAuthor | Hwang I. | - |
dc.identifier.scopusid | 2-s2.0-85084050870 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BACILLE CALMETTE-GUERIN | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODY | - |
dc.subject.keywordPlus | LECTIN RECEPTORS | - |
dc.subject.keywordPlus | FUSION PROTEIN | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | CARBOHYDRATE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | ADJUVANT | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Mycobacterium tuberculosis | - |
dc.subject.keywordAuthor | Nicotiana benthamiana | - |
dc.subject.keywordAuthor | glycosylation | - |
dc.subject.keywordAuthor | Ag85A | - |
dc.subject.keywordAuthor | Th1 response | - |
dc.subject.keywordAuthor | subunit vaccine | - |
dc.subject.keywordAuthor | vaccine antigen | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
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