DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jiyoon | - |
dc.contributor.author | Cho, Siwoo | - |
dc.contributor.author | Park, Yonghyun | - |
dc.contributor.author | Lee, Jiyoul | - |
dc.contributor.author | Park, Jaesung | - |
dc.date.accessioned | 2022-02-22T13:40:07Z | - |
dc.date.available | 2022-02-22T13:40:07Z | - |
dc.date.created | 2022-02-22 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/109447 | - |
dc.description.abstract | <jats:p>Extracellular vesicles (EVs) contain various types of molecules including micro-RNAs, so isolating EVs can be an effective way to analyze and diagnose diseases. A lot of micro-RNAs have been known in relation to prostate cancer (PCa), and we evaluate miR-21, miR-141, and miR-221 in EVs and compare them with prostate-specific antigen (PSA). EVs were isolated from plasma of 38 patients with prostate cancer and 8 patients with benign prostatic hyperplasia (BPH), using a method that showed the highest recovery of RNA. Isolation of EVs concentrated micro-RNAs, reducing the cycle threshold (Ct) value of RT-qPCR amplification of micro-RNA such as miR-16 by 5.12 and miR-191 by 4.65, compared to the values before EV isolation. Normalization of target micro-RNAs was done using miR-191. For miR-221, the mean expression level of patients with localized PCa was significantly higher than that of the control group, having 33.45 times higher expression than the control group (p < 0.01). Area under curve (AUC) between BPH and PCa for miR-221 was 0.98 (p < 0.0001), which was better than AUC for prostate-specific antigen (PSA) level in serum for the same patients. The levels of miR-21 and miR-141 in EVs did not show significant changes in patients with PCa compared to the control group in this study. This study suggests isolating EVs can be a helpful approach in analyzing micro-RNAs with regard to disease.</jats:p> | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLoS ONE | - |
dc.title | Evaluation of micro-RNA in extracellular vesicles from blood of patients with prostate cancer | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0262017 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLoS ONE, v.16, no.12, pp.e0262017 | - |
dc.identifier.wosid | 000773555700083 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | e0262017 | - |
dc.citation.title | PLoS ONE | - |
dc.citation.volume | 16 | - |
dc.contributor.affiliatedAuthor | Park, Jaesung | - |
dc.identifier.scopusid | 2-s2.0-85122038230 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PCR DATA | - |
dc.subject.keywordPlus | CIRCULATING MICRORNAS | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | MIRNAS | - |
dc.subject.keywordPlus | NORMALIZATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | EXOSOMES | - |
dc.subject.keywordPlus | GENES | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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