In vivo and in vitro approaches to identify the molecular and neurodevelopmental function of Zfp536
- Title
- In vivo and in vitro approaches to identify the molecular and neurodevelopmental function of Zfp536
- Authors
- BAEK, SEUNG TAE; 강현구; 장리율
- Date Issued
- 2021-05-20
- Publisher
- 한국뇌신경과학회
- Abstract
- Zfp536 is a zinc finger protein predominantly expressed in developing central
nervous system. Recent genome-wide association studies have identified
Zfp536 as one of the schizophrenia-associated genetic loci. In zebrafish, it
has been shown that Zfp536 functions as a transcription factor implicated
in social behavior and stress. However, its function in developing brain is
not well studied yet. We have adopted systematic approaches to examine
the functions of Zfp536 as a transcription factor in developing brain. First,
we have generated Zfp536 knockout mouse line using CRISPR-Cas9 by the
introduction of a frameshift mutation in exon 3 causing early termination.
Zfp536-/- mouse showed neonatal lethality suggesting its essential role
during embryonic development. We are currently examined the neurodevelopmental
defects of Zfp536-/- and the cause of their death. Second, we have
generated human neuroblastoma cell line (IMR-32) expressing GFP-tagged
ZNF536, a human homologue of mouse Zfp536. By ChIP-seq analysis, we
aim to identify the genome-wide DNA binding sites which may reveal the
function of Zfp536 as a transcription factor. Our approaches will reveal
molecular functions of Zfp536 in developing brain.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/109776
- Article Type
- Conference
- Citation
- KSBNS 2021, 2021-05-20
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