DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dahhan, Dana A | - |
dc.contributor.author | Reynolds, Gregory D | - |
dc.contributor.author | Cárdenas, Jessica J | - |
dc.contributor.author | Eeckhout, Dominique | - |
dc.contributor.author | Johnson, Alexander | - |
dc.contributor.author | Yperman, Klaas | - |
dc.contributor.author | Kaufmann, Walter A | - |
dc.contributor.author | Vang, Nou | - |
dc.contributor.author | Yan, Xu | - |
dc.contributor.author | Hwang, Inhwan | - |
dc.contributor.author | Heese, Antje | - |
dc.contributor.author | De Jaeger, Geert | - |
dc.contributor.author | Friml, Jiri | - |
dc.contributor.author | Van Damme, Daniel | - |
dc.contributor.author | Pan, Jianwei | - |
dc.contributor.author | Bednarek, Sebastian Y | - |
dc.date.accessioned | 2022-03-03T08:40:06Z | - |
dc.date.available | 2022-03-03T08:40:06Z | - |
dc.date.created | 2022-03-03 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 1040-4651 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/110304 | - |
dc.description.abstract | In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from the cell surface as well as the movement of cargo in post-Golgi trafficking pathways. This diversity of functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment and cargo selectivity. The adaptor-protein AP-1 complex operates as part of the secretory pathway at the trans-Golgi network, while the AP-2 complex and the TPLATE complex (TPC) jointly operate at the plasma membrane to execute clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated trafficking in plants will be the comprehensive identification and characterization of the network of evolutionarily conserved and plant-specific core and accessory machinery involved in the formation and targeting of CCVs. To facilitate these studies, we have analyzed the proteome of enriched trans-Golgi network/early endosome-derived and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis (Arabidopsis thaliana) cells. Tandem mass spectrometry analysis results were validated by differential chemical labeling experiments to identify proteins co-enriching with CCVs. Proteins enriched in CCVs included previously characterized CCV components and cargos such as the vacuolar sorting receptors in addition to conserved and plant-specific components whose function in clathrin-mediated trafficking has not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis CCVs is further supported via additional biochemical data. | - |
dc.language | English | - |
dc.publisher | American Society of Plant Biologists | - |
dc.relation.isPartOf | Plant Cell | - |
dc.title | Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/plcell/koac071 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Plant Cell, v.34, no.6, pp.1 - 48 | - |
dc.identifier.wosid | 000767438800001 | - |
dc.citation.endPage | 48 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Plant Cell | - |
dc.citation.volume | 34 | - |
dc.contributor.affiliatedAuthor | Hwang, Inhwan | - |
dc.identifier.scopusid | 2-s2.0-85131107656 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TRANS-GOLGI NETWORK | - |
dc.subject.keywordPlus | VACUOLAR SORTING RECEPTOR | - |
dc.subject.keywordPlus | DOMAIN-CONTAINING PROTEINS | - |
dc.subject.keywordPlus | MEDIATED ENDOCYTOSIS | - |
dc.subject.keywordPlus | ADAPTER COMPLEX | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | CELL PLATE | - |
dc.subject.keywordPlus | SUBCELLULAR-LOCALIZATION | - |
dc.subject.keywordPlus | SECRETORY TRAFFICKING | - |
dc.subject.keywordPlus | DEPENDENT TRAFFICKING | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Cell Biology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.