DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김수란 | en_US |
dc.date.accessioned | 2014-12-01T11:47:18Z | - |
dc.date.available | 2014-12-01T11:47:18Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.other | OAK-2014-00609 | en_US |
dc.identifier.uri | http://postech.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000000900713 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/1111 | - |
dc.description | Master | en_US |
dc.description.abstract | Ligand-receptor interactions on the plasma membrane initiate cellular events. The interactions in the cellular processes may be heterogeneous and dynamic. Such a heterogeneity and dynamics can be revealed through the real time observation at the single-molecule level. Total internal reflection fluorescence (TIRF) microscope allows us to track individual membrane proteins with a high spatio-temporal resolution by reducing autofluorescence from cell. I used aptamer, a modified single stranded DNA, to label a membrane protein in live cells with Quantum dot (Qdot) that is a semiconductor but widely used as a fluorophore for bio-imaging and bio-sensors and that is highly resistant to photo-bleaching. Aptamer can specifically recognize a protein with a high affinity and specificity and is superior to antibody: less interruption of a ligand binding due to a smaller size (< 30 kDa), reproducible production with a DNA synthesizer, and chemically easy modification. However the use of aptamer gave rise to a nonspecific binding to the PLL coated glass of the cell chamber. The utilization of a bare glass and Dextran dramatically suppressed the nonspecific binding of aptamer. The aptamer-Quantum dot complex was successfully applied for following individual insulin receptors in real time. I could not observe a significant change of the Brownian motion and the diffusion coefficient of the insulin receptors after adding insulin as a ligand. However cholesterol depletion of the plasma membrane by methyl-β-cyclodextrin decreases the diffusion coefficient of insulin receptors. It might be thought that the depletion of cholesterol increases the stiffness of the membrane by changing liquid ordered phase (cholesterol-sphingolipid complexes) to quasi-solid phase (sphingolipids). | en_US |
dc.language | kor | en_US |
dc.publisher | 포항공과대학교 | en_US |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | 압타머와 양자점 복합체를 이용한 단일 플라즈마 막 단백질의 동역학 연구 | en_US |
dc.title.alternative | Single-particle tracking of plasma membrane proteins using aptamer-Qdot labeling | en_US |
dc.type | Thesis | en_US |
dc.contributor.college | 일반대학원 물리학과 | en_US |
dc.date.degree | 2011- 2 | en_US |
dc.type.docType | Thesis | - |
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