Stable sample delivery in a viscous medium via a polyimide-based single-channel microfluidic chip for serial crystallography
SCIE
SCOPUS
- Title
- Stable sample delivery in a viscous medium via a polyimide-based single-channel microfluidic chip for serial crystallography
- Authors
- Nam, Ki Hyun; Cho, Yunje
- Date Issued
- 2021-08
- Publisher
- INT UNION CRYSTALLOGRAPHY
- Abstract
- Serial crystallography (SX) provides room-temperature crystal structures with minimal radiation damage and facilitates the comprehension of molecular dynamics through time-resolved studies. In SX experiments, it is important to deliver a large number of crystal samples to the X-ray interaction point in a serial and stable manner. The advantage of crystal delivery in a viscous medium via a capillary is the ability to deliver all of the crystal samples to the X-ray interaction point at a low flow rate; however, the capillary often breaks during handling and high X-ray absorption can occur at low energy states. This study aimed to develop a stable system for sample delivery in a viscous medium via a polyimide-based single-channel microfluidic (PSM) chip for SX. Since this microfluidic chip comprises a polyimide film, it has high tensile strength and higher X-ray transmittance than a quartz capillary. The PSM chip was connected to a syringe containing the microcrystals embedded in viscous medium. The channel of the PSM chip was aligned to the X-ray path, and the viscous medium containing lysozyme crystals was stably delivered using a syringe pump at a flow rate of 100 nl min(-1). Room-temperature lysozyme crystal structures were successfully determined at 1.85 angstrom resolution. This method would greatly facilitate sample delivery for SX experiments using synchrotron X-rays.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/112445
- DOI
- 10.1107/S1600576721005720
- ISSN
- 0021-8898
- Article Type
- Article
- Citation
- JOURNAL OF APPLIED CRYSTALLOGRAPHY, vol. 54, page. 1081 - 1087, 2021-08
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