New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection
SCIE
SCOPUS
- Title
- New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection
- Authors
- Semikolenova, O. A.; Golyshev, V. M.; Kim, B. H.; Venyaminova, A. G.; Novopashina, D. S.
- Date Issued
- 2021-03
- Publisher
- MAIK NAUKA/INTERPERIODICA/SPRINGER
- Abstract
- New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the (Py)A-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-O-methylribonucleotides) that contain either deoxyriboadenosine (dAdA(Py)AdAdA) or the (2'-O-methylribo)adenosine (A(m)A(m Py)AA(m)A(m)) cluster with those of (dAdA(Py)AdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/113307
- DOI
- 10.1134/S1068162021020230
- ISSN
- 1068-1620
- Article Type
- Article
- Citation
- RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, vol. 47, no. 2, page. 432 - 440, 2021-03
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.