DC Field | Value | Language |
---|---|---|
dc.contributor.author | LEE, KEONDO | - |
dc.contributor.author | KIM, JIHAN | - |
dc.contributor.author | BAEK, SANGWON | - |
dc.contributor.author | PARK, JAEHYUN | - |
dc.contributor.author | Park, Sehan | - |
dc.contributor.author | LEE, JONG LAM | - |
dc.contributor.author | CHUNG, WAN KYUN | - |
dc.contributor.author | CHO, YUNJE | - |
dc.contributor.author | NAM, KI HYUN | - |
dc.date.accessioned | 2022-09-06T06:20:23Z | - |
dc.date.available | 2022-09-06T06:20:23Z | - |
dc.date.created | 2022-08-29 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 0021-8898 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/113656 | - |
dc.description.abstract | © 2022 International Union of Crystallography. All rights reserved.Serial femtosecond crystallography (SFX) enables the determination of room-temperature crystal structures of macromolecules with minimized radiation damage and provides time-resolved molecular dynamics by pump-probe or mix-and-inject experiments. In SFX, a variety of sample delivery methods with unique advantages have been developed and applied. The combination of existing sample delivery methods can enable a new approach to SFX data collection that combines the advantages of the individual methods. This study introduces a combined inject-and-transfer system (BITS) method for sample delivery in SFX experiments: a hybrid injection and fixed-target scanning method. BITS allows for solution samples to be reliably deposited on ultraviolet ozone (UVO)-treated polyimide films, at a minimum flow rate of 0.5 nl min-1, in both vertical and horizontal scanning modes. To utilize BITS in SFX experiments, lysozyme crystal samples were embedded in a viscous lard medium and injected at flow rates of 50-100 nl min-1through a syringe needle onto a UVO-treated polyimide film, which was mounted on a fixed-target scan stage. The crystal samples deposited on the film were raster scanned with an X-ray free electron laser using a motion stage in both horizontal and vertical directions. Using the BITS method, the room-temperature structure of lysozyme was successfully determined at a resolution of 2.1 Å, and thus BITS could be utilized in future SFX experiments. | - |
dc.language | English | - |
dc.publisher | Blackwell Publishing Inc. | - |
dc.relation.isPartOf | Journal of Applied Crystallography | - |
dc.title | Combination of an inject-and-transfer system for serial femtosecond crystallography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1107/S1600576722005556 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Journal of Applied Crystallography, v.25, pp.813 - 822 | - |
dc.identifier.wosid | 000837727900011 | - |
dc.citation.endPage | 822 | - |
dc.citation.startPage | 813 | - |
dc.citation.title | Journal of Applied Crystallography | - |
dc.citation.volume | 25 | - |
dc.contributor.affiliatedAuthor | LEE, KEONDO | - |
dc.contributor.affiliatedAuthor | KIM, JIHAN | - |
dc.contributor.affiliatedAuthor | BAEK, SANGWON | - |
dc.contributor.affiliatedAuthor | PARK, JAEHYUN | - |
dc.contributor.affiliatedAuthor | LEE, JONG LAM | - |
dc.contributor.affiliatedAuthor | CHUNG, WAN KYUN | - |
dc.contributor.affiliatedAuthor | CHO, YUNJE | - |
dc.contributor.affiliatedAuthor | NAM, KI HYUN | - |
dc.identifier.scopusid | 2-s2.0-85135633896 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | serial crystallography | - |
dc.subject.keywordAuthor | sample delivery | - |
dc.subject.keywordAuthor | injection | - |
dc.subject.keywordAuthor | fixed-target scanning | - |
dc.subject.keywordAuthor | viscous media | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Crystallography | - |
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