DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, Sun Ju | - |
dc.contributor.author | Song, Woo Chul | - |
dc.contributor.author | Jung, Sung Hwan | - |
dc.contributor.author | Cho, Seung-Woo | - |
dc.contributor.author | Kim, Dong-Ik | - |
dc.contributor.author | Um, Soong Ho | - |
dc.date.accessioned | 2023-02-28T09:20:39Z | - |
dc.date.available | 2023-02-28T09:20:39Z | - |
dc.date.created | 2023-02-28 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 0743-7463 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/116036 | - |
dc.description.abstract | A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in various parameters: the number of gene-networked gel (G-net-gel) spheroids, the concentration of a AcGFP plasmid in the scaffold, and the molar ratio between the X-DNA building blocks and the gene. It was then assessed that 800 units of the gene networked gel matrix at a 4000:1 molar ratio of X-DNA blocks and AcGFP gene components accomplished 20-fold enhanced in vitro protein expression efficiency for 36 h. Furthermore, once with lipid capping, it reproduced the natural nucleus system, demonstrating the 2-fold increased levels of messenger RNAs (mRNAs) relative to solution phase vectors. | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | Langmuir | - |
dc.title | A Gene-Networked Gel Matrix-Supported Lipid Bilayer as a Synthetic Nucleus System | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/la303498k | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Langmuir, v.28, no.49, pp.17036 - 17042 | - |
dc.identifier.wosid | 000312122300038 | - |
dc.citation.endPage | 17042 | - |
dc.citation.number | 49 | - |
dc.citation.startPage | 17036 | - |
dc.citation.title | Langmuir | - |
dc.citation.volume | 28 | - |
dc.contributor.affiliatedAuthor | Song, Woo Chul | - |
dc.identifier.scopusid | 2-s2.0-84870955378 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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