Reduction in CO2 uptake rates of red tide dinoflagellates due to mixotrophy

Abstract

We investigated a possible reduction in CO2 uptake rate by phototrophic red tide dinoflagellates arising from mixotrophy. We measured the daily ingestion rates of Prorocentrum minimum by Prorocentrum micans over 5 days in 10 L experimental bottles, and the uptake rates of total dissolved inorganic carbon (CT) by a mixture of P. micans and P. minimum (mixotrophic growth), and for the predator P. micans (phototrophic growth; control) and prey P. minimum (phototrophic growth; control) alone. To account for the effect of pH on the phototrophic growth rates of P. micans and P. minimum, measurements of CTand pH in the predator and prey control bottles were continued until the pH reached the same level (pH 9.5) as that in the experimental bottles on the final day of incubation. The measured total CTuptake rate by the mixture of P. micans and P. minimum changed from 123 to 161 μmol CTkg-1d-1over the course of the experiment, and was lower than the CTuptake rates shown by P. micans and P. minimum in the predator and prey control bottles, respectively, which changed from 132 to 176 μmol CTkg-1d-1over the course of the experiment. The reduction in total CTuptake rate arising from the mixotrophy of P. micans was 7-31% of the daily CTuptake rate seen during photosynthesis. The results suggest that red tide dinoflagellates take up less CTduring mixotrophy. © 2016 The Korean Society of Phycology.