DC Field | Value | Language |
---|---|---|
dc.contributor.author | Akter, shammi | - |
dc.date.accessioned | 2023-08-31T16:30:26Z | - |
dc.date.available | 2023-08-31T16:30:26Z | - |
dc.date.issued | 2023 | - |
dc.identifier.other | OAK-2015-10018 | - |
dc.identifier.uri | http://postech.dcollection.net/common/orgView/200000660028 | ko_KR |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/118215 | - |
dc.description | Doctor | - |
dc.description.abstract | GLP-1 (Glucagon-like peptide-1) is a peptide that stimulates insulin secretion from the β-cell for glycemic control of the plasma blood glucose level. Its mimetic exenatide (synthetic Exendin-4) with a longer half-life of approximately 3.3–4 h is widely used in clinical application to treat diabetes. Currently, exenatide is chemically synthesized. In this study, we report that the GLP-1 analogue recombinant Exendin-4 (Exdn-4) can be produced at a high level in Nicotiana benthamiana, with an estimated yield of 50.0 µg/g fresh biomass. For high-level expression, we generated a recombinant gene, B:GB1:ddCBD1m:8xHis:Exendin-4 (BGC:Exdn-4), for the production of Exendin-4 using various domains such as the BiP signal peptide sequence, the B1 domain of streptococcal G protein (GB1), a double cellulose binding domain 1 (CBD1), and 8 His residues (8xHis) to the N-terminus of Exendin-4. GB1 was used to increase the expression, whereas double CBD1 and 8xHis were included as affinity tags for easy purification using Microcrystalline cellulose and Ni2+-NTA resin, respectively. BGC:Exdn-4 was purified by single-step purification to near homogeneity using both Ni2+-NTA resin and microcrystalline cellulose beads. Moreover, Exdn-4 without any extra residues was produced from BGC:Exdn-4 bound onto MCC beads by treating with enterokinase. Plant-produced Exdn-4 (Exendin-4) was as effective as chemically synthesized Exendin-4 in glucose-induced insulin secretion (GIIS) from mouse MIN6m9 cells a pancreatic beta cell line. | - |
dc.language | eng | - |
dc.publisher | 포항공과대학교 | - |
dc.title | Production of active Exendin-4 in Nicotiana benthamiana and its application in treatment of type-2 diabetics | - |
dc.type | Thesis | - |
dc.contributor.college | 생명과학과 | - |
dc.date.degree | 2023- 2 | - |
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