DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho Eunbyul | - |
dc.contributor.author | Woo Youngsik | - |
dc.contributor.author | Suh Yeongjun | - |
dc.contributor.author | Suh Bo Kyoung | - |
dc.contributor.author | Kim Soo Jeong | - |
dc.contributor.author | Nhung Truong Thi My | - |
dc.contributor.author | Yoo Jin Yeong | - |
dc.contributor.author | Nghi Tran Diem | - |
dc.contributor.author | Lee Su Been | - |
dc.contributor.author | Mun Dong Jin | - |
dc.contributor.author | Park Sang Ki | - |
dc.date.accessioned | 2023-09-04T00:21:09Z | - |
dc.date.available | 2023-09-04T00:21:09Z | - |
dc.date.created | 2023-09-01 | - |
dc.date.issued | 2023-06 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/118757 | - |
dc.description.abstract | Mitochondria-associated ER membrane (MAM) is a structure where these calcium-regulating organelles form close physical contact sites for efficient Ca2+ crosstalk. Despite the central importance of MAM Ca2+ dynamics in diverse biological processes, directly and specifically measuring Ca2+ concentrations inside MAM is technically challenging. Here, we develop MAM-Calflux, a MAM-specific BRET-based Ca2+ indicator. The successful application of the bimolecular fluorescence complementation (BiFC) concept highlights Ca2+-responsive BRET signals in MAM. The BiFC strategy imparts dual functionality as a Ca2+ indicator and quantitative structural marker specific for MAM. As a ratiometric Ca2+ indicator, MAM-Calflux estimates steady-state MAM Ca2+ levels. Finally, it enables the visualization of uneven intracellular distribution of MAM Ca2+ and the elucidation of abnormally accumulated MAM Ca2+ from the neurons of Parkinson's disease mouse model in both steady-state and stimulated conditions. Therefore, we propose that MAM-Calflux can be a versatile tool for ratiometrically measuring dynamic inter-organellar Ca2+ communication.,MAM Ca2+ dynamics play an important role in diverse biological processes, but directly and specifically measuring Ca2+ concentrations in this region is technically challenging. Here the authors report a MAM-specific BRET-based Ca2+ indicator called MAM-Calflux, which works as both a Ca2+ indicator and a structural marker due to its ratiometric nature., | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | Nature Communications | - |
dc.title | Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41467-023-39343-2 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Nature Communications, v.14, no.1 | - |
dc.identifier.wosid | 001018392800027 | - |
dc.citation.number | 1 | - |
dc.citation.title | Nature Communications | - |
dc.citation.volume | 14 | - |
dc.contributor.affiliatedAuthor | Park Sang Ki | - |
dc.identifier.scopusid | 2-s2.0-85162023606 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | FLUORESCENCE COMPLEMENTATION ASSAY | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM CA2+ | - |
dc.subject.keywordPlus | DEPENDENT ANION CHANNEL | - |
dc.subject.keywordPlus | ALPHA-SYNUCLEIN | - |
dc.subject.keywordPlus | TRANSGENIC MICE | - |
dc.subject.keywordPlus | MITOCHONDRIAL | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | SITES | - |
dc.subject.keywordPlus | BIOENERGETICS | - |
dc.subject.keywordPlus | VISUALIZATION | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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