A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2
SCIE
SCOPUS
- Title
- A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2
- Authors
- Lee, Ae Sol; KIm, Su Min; KIm, Kyeong Rok; Park, Chulmin; Lee, Dong-Gun; Heo, Hye Ryoung; Cha, Hyung Joon; Kim, Chang Sup
- Date Issued
- 2023-03
- Publisher
- Elsevier BV
- Abstract
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19). The high human-to-human transmission and rapid evolution of SARS-CoV-2 have resulted in a worldwide pandemic. To contain SARS-CoV-2, it is essential to efficiently control the transmission of the virus through the early diagnosis of infected individuals, including asymptomatic people. Therefore, a rapid and accurate assay is vital for the early diagnosis of SARS-CoV-2 in suspected individuals. In this study, we developed a colorimetric lateral flow immunoassay (LFIA) in which a CBP31-BC linker was used to immobilize antibodies on a cellulose membrane in an oriented manner. The developed LFIA enabled sensitive detection of cultured SARS-CoV-2 in 15 min with a detection limit of 5 x 104 copies/mL. The clinical performance of the LFIA for detecting SARS-CoV-2 was evaluated using 19 clinical samples validated by reverse transcription-polymerase chain reaction (RT-PCR). The LFIA detected all the positive and negative samples accurately, corresponding to 100% accuracy. Impor-tantly, patient samples with low viral loads were accurately identified. Thus, the proposed method can provide a useful platform for rapid and accurate point-of-care testing of SARS-CoV-2 in infected individuals to efficiently control the COVID-19 pandemic.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/120748
- DOI
- 10.1016/j.snb.2022.133245
- Article Type
- Article
- Citation
- Sensors and Actuators, B: Chemical, vol. 379, 2023-03
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- There are no files associated with this item.
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