Studying plant autophagy: challenges and recommended methodologies
- Title
- Studying plant autophagy: challenges and recommended methodologies
- Authors
- Qi, Hua; Wang, Yao; Bao, Yan; Bassham, Diane C.; Chen, Liang; Chen, Qin-Fang; Hou, Suiwen; Hwang, Inhwan; Huang, Li; Lai, Zhibing; Li, Faqiang; Liu, Yule; Qiu, Rongliang; Wang, Hao; Wang, Pengwei; Xie, Qingjun; Zeng, Yonglun; Zhuang, Xiaohong; Gao, Caiji; Jiang, Liwen; Xiao, Shi
- Date Issued
- 2023-10
- Publisher
- Springer Science and Business Media LLC
- Abstract
- AbstractIn plants, autophagy is a conserved process by which intracellular materials, including damaged proteins, aggregates, and entire organelles, are trafficked to the vacuole for degradation, thus maintaining cellular homeostasis. The past few decades have seen extensive research into the core components of the central autophagy machinery and their physiological roles in plant growth and development as well as responses to biotic and abiotic stresses. Moreover, several methods have been established for monitoring autophagic activities in plants, and these have greatly facilitated plant autophagy research. However, some of the methodologies are prone to misuse or misinterpretation, sometimes casting doubt on the reliability of the conclusions being drawn about plant autophagy. Here, we summarize the methods that are widely used for monitoring plant autophagy at the physiological, microscopic, and biochemical levels, including discussions of their advantages and limitations, to provide a guide for studying this important process.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/120766
- DOI
- 10.1007/s44307-023-00002-8
- ISSN
- 2948-2801
- Article Type
- Article
- Citation
- Advanced Biotechnology, vol. 1, no. 4, 2023-10
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.