DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Eun-Jeong | - |
dc.contributor.author | Hwang, Seong-Hye | - |
dc.contributor.author | Seo, Seungwan | - |
dc.contributor.author | Park, Jaesung | - |
dc.contributor.author | Park, Seokwoo | - |
dc.contributor.author | Kim, Sejoong | - |
dc.date.accessioned | 2024-06-20T05:41:45Z | - |
dc.date.available | 2024-06-20T05:41:45Z | - |
dc.date.created | 2024-04-26 | - |
dc.date.issued | 2023-11 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/123622 | - |
dc.description.abstract | Mesenchymal stromal cell (MSC)-derived extracellular vesicles (EVs) are known to have a therapeutic effect on nephrotoxicity. As animal models require significant time and resources to evaluate drug effects, there is a need for a new experimental technique that can accurately predict drug effects in humans. We evaluated the therapeutic effect of MSC-derived EVs in cisplatin nephrotoxicity using a three-dimensional, gravity-driven, two-layer tubule-on-a-chip (3D-MOTIVE chip). In the 3D-MOTIVE chip, 10 mu M cisplatin decreased the number of attached cells compared to the vehicle. Conversely, annexin V and reactive oxygen species (ROS) were increased. Cell viability was increased 2.8-fold and 2.5-fold after treatment with EVs at 4 and 8 mu g/mL, respectively, compared to the cisplatin-induced nephrotoxicity group. Cell attachment was increased 2.25-fold by treatment with 4 mu g/mL EVs and 2.02-fold by 8 mu g/mL EVs. Annexin V and ROS levels were decreased compared to those in the cisplatin-induced nephrotoxicity group. There were no significant differences in annexin V and ROS levels according to EV concentration. In sum, we created a cisplatin-induced nephrotoxicity model on a 3D-MOTIVE chip and found that MSC-derived EVs could restore cell viability. Thus, MSC-derived EVs may have the potential to ameliorate cisplatin-induced nephrotoxicity. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.title | Efficacy of Mesenchymal-Stromal-Cell-Derived Extracellular Vesicles in Ameliorating Cisplatin Nephrotoxicity, as Modeled Using Three-Dimensional, Gravity-Driven, Two-Layer Tubule-on-a-Chip (3D-MOTIVE Chip) | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms242115726 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.24, no.21 | - |
dc.identifier.wosid | 001099481900001 | - |
dc.citation.number | 21 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Park, Jaesung | - |
dc.identifier.scopusid | 2-s2.0-85176459312 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ACUTE KIDNEY INJURY | - |
dc.subject.keywordPlus | EXOSOME | - |
dc.subject.keywordAuthor | extracellular vesicles (EVs) | - |
dc.subject.keywordAuthor | acute kidney injury | - |
dc.subject.keywordAuthor | tubule-on-a-chip | - |
dc.subject.keywordAuthor | 3D-MOTIVE chip | - |
dc.subject.keywordAuthor | drug efficacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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