Imaging live cell in micro-liquid enclosure by X-ray laser diffraction
SCIE
SCOPUS
- Title
- Imaging live cell in micro-liquid enclosure by X-ray laser diffraction
- Authors
- Kimura, T; Joti, Y; Shibuya, A; Song, CY; Kim, S; Tono, K; Yabashi, M; Tamakoshi, M; Moriya, T; Oshima, T; Ishikawa, T; Bessho, Y; Nishino, Y
- Date Issued
- 2014-01
- Publisher
- NPG
- Abstract
- Emerging X-ray free-electron lasers with femtosecond pulse duration enable single-shot snapshot imaging almost free from sample damage by outrunning major radiation damage processes. In bioimaging, it is essential to keep the sample close to its natural state. Conventional high-resolution imaging, however, suffers from severe radiation damage that hinders live cell imaging. Here we present a method for capturing snapshots of live cells kept in a micro-liquid enclosure array by X-ray laser diffraction. We place living Microbacterium lacticum cells in an enclosure array and successively expose each enclosure to a single X-ray laser pulse from the SPring-8 Angstrom Compact Free-Electron Laser. The enclosure itself works as a guard slit and allows us to record a coherent diffraction pattern from a weakly-scattering submicrometre-sized cell with a clear fringe extending up to a 28-nm full-period resolution. The reconstructed image reveals living whole-cell structures without any staining, which helps advance understanding of intracellular phenomena.
- Keywords
- FREE-ELECTRON LASER; ATOMIC-RESOLUTION; SCATTERING; CRYSTALLOGRAPHY; SPECIMENS; BACTERIA; WHOLE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13282
- DOI
- 10.1038/NCOMMS4052
- ISSN
- 2041-1723
- Article Type
- Article
- Citation
- NATURE COMMUNICATIONS, vol. 5, page. 3052, 2014-01
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