DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rodriguez, JA | - |
dc.contributor.author | Xu, R | - |
dc.contributor.author | Chen, CC | - |
dc.contributor.author | Huang, ZF | - |
dc.contributor.author | Jiang, HD | - |
dc.contributor.author | Chen, AL | - |
dc.contributor.author | Raines, KS | - |
dc.contributor.author | Pryor, A | - |
dc.contributor.author | Nam, D | - |
dc.contributor.author | Wiegart, L | - |
dc.contributor.author | Song, C | - |
dc.contributor.author | Madsen, A | - |
dc.contributor.author | Chushkin, Y | - |
dc.contributor.author | Zontone, F | - |
dc.contributor.author | Bradley, PJ | - |
dc.contributor.author | Miao, JW | - |
dc.date.accessioned | 2016-01-08T14:52:04Z | - |
dc.date.available | 2016-01-08T14:52:04Z | - |
dc.date.created | 2015-09-14 | - |
dc.date.issued | 2015-09 | - |
dc.identifier.issn | 2052-2525 | - |
dc.identifier.other | 2015-OAK-0000033543 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/13467 | - |
dc.description.abstract | A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the threedimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres. | - |
dc.description.statementofresponsibility | open | - |
dc.language | English | - |
dc.publisher | IUCr | - |
dc.relation.isPartOf | IUCRJ | - |
dc.title | Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells | - |
dc.type | Article | - |
dc.contributor.college | 물리학과 | - |
dc.identifier.doi | 10.1107/S205225251501235X | - |
dc.author.google | Rodriguez, JA | - |
dc.author.google | Xu, R | - |
dc.author.google | Chen, CC | - |
dc.author.google | Huang, ZF | - |
dc.author.google | Jiang, HD | - |
dc.author.google | Chen, AL | - |
dc.author.google | Raines, KS | - |
dc.author.google | Pryor, A | - |
dc.author.google | Nam, D | - |
dc.author.google | Wiegart, L | - |
dc.author.google | Song, C | - |
dc.author.google | Madsen, A | - |
dc.author.google | Chushkin, Y | - |
dc.author.google | Zontone, F | - |
dc.author.google | Bradley, PJ | - |
dc.author.google | Miao, JW | - |
dc.relation.volume | 2 | - |
dc.relation.issue | 5 | - |
dc.relation.startpage | 575 | - |
dc.relation.lastpage | 583 | - |
dc.contributor.id | 10084037 | - |
dc.relation.journal | IUCRJ | - |
dc.relation.sci | SCIE | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | IUCRJ, v.2, no.5, pp.575 - 583 | - |
dc.identifier.wosid | 000360067300013 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 583 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 575 | - |
dc.citation.title | IUCRJ | - |
dc.citation.volume | 2 | - |
dc.contributor.affiliatedAuthor | Song, C | - |
dc.identifier.scopusid | 2-s2.0-84940921046 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 30 | - |
dc.description.scptc | 36 | * |
dc.date.scptcdate | 2018-10-274 | * |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RED-BLOOD-CELLS | - |
dc.subject.keywordPlus | TOXOPLASMA-GONDII | - |
dc.subject.keywordPlus | ELECTRON TOMOGRAPHY | - |
dc.subject.keywordPlus | PHASE RETRIEVAL | - |
dc.subject.keywordPlus | LIQUID-NITROGEN | - |
dc.subject.keywordPlus | MICROSCOPY | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | CRYSTALLOGRAPHY | - |
dc.subject.keywordPlus | SPECIMENS | - |
dc.subject.keywordPlus | ULTRASTRUCTURE | - |
dc.subject.keywordAuthor | coherent diffractive imaging | - |
dc.subject.keywordAuthor | cryo-CDI | - |
dc.subject.keywordAuthor | three-dimensional imaging | - |
dc.subject.keywordAuthor | three-dimensional cellular structure | - |
dc.subject.keywordAuthor | coherent diffraction | - |
dc.subject.keywordAuthor | X-ray imaging | - |
dc.subject.keywordAuthor | Neospora caninum | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalResearchArea | Materials Science | - |
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