DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, JH | - |
dc.contributor.author | Kim, JH | - |
dc.contributor.author | Kim, EY | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Song, HY | - |
dc.contributor.author | Kim, WJ | - |
dc.contributor.author | Lee, D | - |
dc.contributor.author | Park, J | - |
dc.contributor.author | Kim, S | - |
dc.date.accessioned | 2016-03-31T07:39:10Z | - |
dc.date.available | 2016-03-31T07:39:10Z | - |
dc.date.created | 2015-02-04 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 0033-8419 | - |
dc.identifier.other | 2014-OAK-0000031611 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/13865 | - |
dc.description.abstract | To evaluate the effectiveness of small interfering RNA (siRNA) targeting matrix metalloproteinase 9 (MMP-9) in suppressing granulation tissue formation caused by bare metallic stent placement in a rat urethral model. All experiments were approved by the committee of animal research. In 20 Sprague-Dawley male rats (weight range, 300-350 g), a self-expanding metallic bare stent was inserted in the urethra with fluoroscopic guidance. One group of 10 rats (group A) was treated with MMP-9 siRNA/bioreducible branched polyethylenimine-disulfide cross-linked-indocyanine green (bioreducible BPEI-SS-ICG), while the other group of 10 rats (group B) received control siRNA/bioreducible BPEI-SS-ICG treatment. All rats were sacrificed at 4 weeks. The therapeutic effectiveness of the MMP-9 siRNA/bioreducible BPEI-SS-ICG complex was assessed by comparing the two results of retrograde urethrography, histologic examination, and quantification of MMP-9 by using zymography and Western blot analysis between the two groups. The Mann-Whitney U test was used to evaluate differences. Stent placement was successful in all rats without a single case of migration at follow-up. Retrograde urethrography performed 4 weeks after stent placement demonstrated significantly larger luminal diameters of the urethra within the stents in group A compared with those in group B (P = .011). Histologic analysis revealed that the mean percentage of granulation tissue area (P < .001), mean number of epithelial layers (P < .001), and mean thickness of submucosal fibrosis (P < .001) were significantly decreased in group A compared with group B. Meanwhile, the mean density of inflammatory cell infiltration did not significantly differ between the two groups (P = .184). Quantitative analysis disclosed MMP-9 levels to be lower in group A relative to group B, indicating positive inhibition of MMP-9 by MMP-9 siRNA/bioreducible BPEI-SS-ICG. MMP-9 siRNA/bioreducible BPEI-SS-ICG is effective for inhibiting granulation tissue formation after bare metallic stent placement in a rat urethral model. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Radiological Society of North America | - |
dc.relation.isPartOf | Radiology | - |
dc.title | Bioreducible Polymer-delivered siRNA Targeting MMP-9: Suppression of Granulation Tissue Formation after Bare Metallic Stent Placement in a Rat Urethral Model | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1148/RADIOL.13130980 | - |
dc.author.google | Park, JH | - |
dc.author.google | Kim, JH | - |
dc.author.google | Kim, EY | - |
dc.author.google | Kim, J | - |
dc.author.google | Song, HY | - |
dc.author.google | Kim, WJ | - |
dc.author.google | Lee, D | - |
dc.author.google | Park, J | - |
dc.author.google | Kim, S | - |
dc.relation.volume | 271 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 87 | - |
dc.relation.lastpage | 95 | - |
dc.contributor.id | 10135304 | - |
dc.relation.journal | RADIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Radiology, v.271, no.1, pp.87 - 95 | - |
dc.identifier.wosid | 000335139200009 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 95 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 87 | - |
dc.citation.title | Radiology | - |
dc.citation.volume | 271 | - |
dc.contributor.affiliatedAuthor | Kim, WJ | - |
dc.identifier.scopusid | 2-s2.0-84896972041 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 4 | - |
dc.description.scptc | 3 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | EXPANDABLE NITINOL STENT | - |
dc.subject.keywordPlus | ELUTING COVERED STENT | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | COATED STENTS | - |
dc.subject.keywordPlus | HYPERPLASIA | - |
dc.subject.keywordPlus | PREVENTION | - |
dc.subject.keywordPlus | STRICTURES | - |
dc.subject.keywordPlus | EXPERIENCE | - |
dc.subject.keywordPlus | DYE | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
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