DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, B | - |
dc.contributor.author | Shin, GW | - |
dc.contributor.author | Choi, W | - |
dc.contributor.author | Joo, J | - |
dc.contributor.author | Jeon, S | - |
dc.contributor.author | Jung, GY | - |
dc.date.accessioned | 2016-03-31T07:41:51Z | - |
dc.date.available | 2016-03-31T07:41:51Z | - |
dc.date.created | 2015-02-09 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.other | 2015-OAK-0000031507 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/13914 | - |
dc.description.abstract | Antibody-conjugated magnetic nanoparticles (Ab-MNPs) have potential in pathogen detection because they allow target cells to be easily separated from complex sample matrices. However, the sensitivity and specificity of pathogen capture by Ab-MNPs generally vary according to the types of MNPs, antibodies, and sample matrices, as well as preparation methods, including immobilization. Therefore, achieving a reproducible analysis utilizing Ab-MNPs as a pathogen detection method requires accurate characterization of Ab-MNP capture ability and standardization of all handling processes. In this study, we used high-resolution CE-single strand conformational polymorphism coupled with a stuffer-free multiplex ligation-dependent probe amplification system to characterize Ab-MNPs. The capture ability of Ab-MNPs targeting Salmonella enteritidis and nine pathogens, including S. enteritidis, was analyzed in phosphate buffer and milk. The effect of storage conditions on the stability of Ab-MNPs was also assessed. The results showed that the stuffer-free multiplex ligation-dependent probe amplification system has the potential to serve as a standard characterization method for Ab-MNPs. Moreover, the precise characterization of Ab-MNPs facilitated robust pathogen detection in various applications. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.relation.isPartOf | ELECTROPHORESIS | - |
dc.subject | Antibody-conjugated magnetic nanoparticles | - |
dc.subject | Capillary electrophoresis-single-strand conformation polymorphism | - |
dc.subject | Pathogen detection | - |
dc.subject | Stuffer-free multiplex ligation-dependent probe amplification | - |
dc.subject | RESOLUTION CE-SSCP | - |
dc.subject | ESCHERICHIA-COLI O157-H7 | - |
dc.subject | FOOD SAMPLES | - |
dc.subject | IMPEDANCE BIOSENSOR | - |
dc.subject | SENSITIVE DETECTION | - |
dc.subject | BACTERIA | - |
dc.subject | SEPARATION | - |
dc.subject | SENSOR | - |
dc.subject | PCR | - |
dc.title | Precise characterization method of antibody-conjugated magnetic nanoparticles for pathogen detection using stuffer-free multiplex ligation-dependent probe amplification | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1002/ELPS.201400180 | - |
dc.author.google | Chung, B | - |
dc.author.google | Shin, GW | - |
dc.author.google | Choi, W | - |
dc.author.google | Joo, J | - |
dc.author.google | Jeon, S | - |
dc.author.google | Jung, GY | - |
dc.relation.volume | 35 | - |
dc.relation.issue | 23 | - |
dc.relation.startpage | 3283 | - |
dc.relation.lastpage | 3289 | - |
dc.contributor.id | 10132035 | - |
dc.relation.journal | ELECTROPHORESIS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.35, no.23, pp.3283 - 3289 | - |
dc.identifier.wosid | 000345972900001 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 3289 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 3283 | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 35 | - |
dc.contributor.affiliatedAuthor | Jeon, S | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-84914095557 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 6 | - |
dc.description.scptc | 5 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI O157-H7 | - |
dc.subject.keywordPlus | IMPEDANCE BIOSENSOR | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordAuthor | Antibody-conjugated magnetic nanoparticles | - |
dc.subject.keywordAuthor | Capillary electrophoresis-single-strand conformation polymorphism | - |
dc.subject.keywordAuthor | Pathogen detection | - |
dc.subject.keywordAuthor | Stuffer-free multiplex ligation-dependent probe amplification | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
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