Oxygen-dependent enhancement of hydrogen production by engineering bacterial hemoglobin in Escherichia coli
SCIE
SCOPUS
- Title
- Oxygen-dependent enhancement of hydrogen production by engineering bacterial hemoglobin in Escherichia coli
- Authors
- Jo, BH; Kim, JYH; Seo, JH; Cha, HJ
- Date Issued
- 2014-07-03
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Abstract
- H-2 production under aerobic conditions has been proposed as an alternative method to overcome the fundamentally low yield of H-2 production by fermentative bacteria by maximizing the number of electrons that are available for H-2. Here, we engineered Vitreoscilla hemoglobin (VHb) in Escherichia cob to study the effects of this versatile oxygen (O-2)-binding protein on oxic H-2 production in a closed batch system that was supplemented with glucose. The H-2 yields that were obtained with the VHb-expressing E. coli were greatly enhanced in comparison to the negative control cells in culture that started with high O-2 tensions. The formate hydrogen lyase (FHL) activity of oxically cultured, VHb-expressing cells was also much higher than that of the negative control cells. Through inhibitor studies and time-course experiments, VHb was shown to contribute to the improved H-2 yield primarily by increasing the efficiency of cellular metabolism during the aerobic phase before the onset of H-2 production and not by working as an O-2-scavenger during H-2 production. This new approach allowed more substrate to remain to be further utilized for the production of more H-2 from limited resources. We expect that VHb can be successfully engineered in potential aerobic H-2-producing microbial systems to enhance the overall H-2 production yield. In addition, the remarkably high FHL activity of oxically grown, VHb-expressing cells may make this engineered strain an attractive whole-cell biocatalyst for converting formate to H-2. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved,
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13976
- DOI
- 10.1016/J.IJHYDENE.2014.04.209
- ISSN
- 0360-3199
- Article Type
- Article
- Citation
- INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol. 39, no. 20, page. 10426 - 10433, 2014-07-03
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