PET imaging of stroke-induced neuroinflammation in mice using [18F]PBR06
SCIE
SCOPUS
- Title
- PET imaging of stroke-induced neuroinflammation in mice using [18F]PBR06
- Authors
- Lartey, FM; Ahn, GO; Shen, B; Cord, KT; Smith, T; Chua, JY; Rosenblum, S; Liu, HG; James, ML; Chernikova, S; Lee, SW; Pisani, LJ; Tirouvanziam, R; Chen, JW; Palmer, TD; Chin, FT; Guzman, R; Graves, EE; Loo, BW
- Date Issued
- 2014-02
- Publisher
- Springer
- Abstract
- The purpose of this study is to evaluate the 18 kDa translocator protein (TSPO) radioligand [F-18]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([F-18]PBR06) as a positron emission tomography (PET) imaging biomarker of stroke-induced neuroinflammation in a rodent model. Stroke was induced by transient middle cerebral artery occlusion in Balb/c mice. Dynamic PET/CT imaging with displacement and preblocking using PK111195 was performed 3 days later. PET data were correlated with immunohistochemistry (IHC) for the activated microglial markers TSPO and CD68 and with autoradiography. [F-18]PBR06 accumulation peaked within the first 5 min postinjection, then decreased gradually, remaining significantly higher in infarct compared to noninfarct regions. Displacement or preblocking with PK11195 eliminated the difference in [F-18]PBR06 uptake between infarct and noninfarct regions. Autoradiography and IHC correlated well spatially with uptake on PET. [F-18]PBR06 PET specifically images TSPO in microglial neuroinflammation in a mouse model of stroke and shows promise for imaging and monitoring microglial activation/neuroinflammation in other disease models.
- Keywords
- Stroke; Neuroinflammation; Translocator protein 18 kDa (TSPO); Peripheral benzodiazepine receptor (PBR); Positron emission tomography (PET); [F-18]PBR06; PROTEIN 18 KDA; PERIPHERAL BENZODIAZEPINE-RECEPTOR; POSITRON-EMISSION-TOMOGRAPHY; IN-VIVO; INFLAMMATORY RESPONSE; CEREBRAL-ISCHEMIA; HUMAN BRAIN; RAT-BRAIN; MICROGLIA; LIGAND
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/14869
- DOI
- 10.1007/S11307-013-0664-5
- ISSN
- 1536-1632
- Article Type
- Article
- Citation
- Mol Imaging Biol, vol. 16, no. 1, page. 109 - 117, 2014-02
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