DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SM | - |
dc.contributor.author | Paek, KH | - |
dc.contributor.author | Lee, SB | - |
dc.date.accessioned | 2016-03-31T08:18:54Z | - |
dc.date.available | 2016-03-31T08:18:54Z | - |
dc.date.created | 2014-02-14 | - |
dc.date.issued | 2012-05 | - |
dc.identifier.issn | 1431-0651 | - |
dc.identifier.other | 2012-OAK-0000028807 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15031 | - |
dc.description.abstract | utilizes -rhamnose as a sole carbon source. To determine the metabolic pathway of -rhamnose in Archaea, we identified and characterized -rhamnose dehydrogenase (RhaD) in . Ta0747P gene, which encodes the putative RhaD (Ta_RhaD) enzyme belonging to the short-chain dehydrogenase/reductase family, was expressed in as an active enzyme catalyzing the oxidation of -rhamnose to -rhamnono-1,4-lactone. Analysis of catalytic properties revealed that Ta_RhaD oxidized -rhamnose, -lyxose, and -mannose using only NADP(+) as a cofactor, which is different from NAD(+)/NADP(+)-specific bacterial RhaDs and NAD(+)-specific eukaryal RhaDs. Ta_RhaD showed the highest activity toward -rhamnose at 60 A degrees C and pH 7. The (m) and (cat) values were 0.46 mM, 1,341.3 min(-1) for -rhamnose and 0.1 mM, 1,027.2 min(-1) for NADP(+), respectively. Phylogenetic analysis indicated that branched lineages of archaeal RhaD are quite distinct from those of Bacteria and Eukarya. This is the first report on the identification and characterization of NADP(+)-specific RhaD. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER TOKYO | - |
dc.relation.isPartOf | EXTREMOPHILES | - |
dc.subject | NADP-specific L-rhamnose dehydrogenase | - |
dc.subject | Thermoplasma acidophilum | - |
dc.subject | Thermophilic enzyme | - |
dc.subject | Archaea | - |
dc.subject | Non-phosphorylated pathway | - |
dc.subject | ENTNER-DOUDOROFF PATHWAY | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | SULFOLOBUS-SOLFATARICUS | - |
dc.subject | L-RHAMNULOSE | - |
dc.subject | GLUCOSE-DEHYDROGENASE | - |
dc.subject | COENZYME SPECIFICITY | - |
dc.subject | CARBONYL REDUCTASE | - |
dc.subject | INTRACELLULAR PH | - |
dc.subject | METABOLISM | - |
dc.subject | ALDOLASE | - |
dc.title | Characterization of NADP(+)-specific L-rhamnose dehydrogenase from the thermoacidophilic Archaeon Thermoplasma acidophilum | - |
dc.type | Article | - |
dc.contributor.college | 엔지니어링 대학원 | - |
dc.identifier.doi | 10.1007/S00792-012-0444-1 | - |
dc.author.google | Kim, SM | - |
dc.author.google | Paek, KH | - |
dc.author.google | Lee, SB | - |
dc.relation.volume | 16 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 447 | - |
dc.relation.lastpage | 454 | - |
dc.contributor.id | 10105619 | - |
dc.relation.journal | EXTREMOPHILES | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCIE | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | EXTREMOPHILES, v.16, no.3, pp.447 - 454 | - |
dc.identifier.wosid | 000303870300010 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 454 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 447 | - |
dc.citation.title | EXTREMOPHILES | - |
dc.citation.volume | 16 | - |
dc.contributor.affiliatedAuthor | Lee, SB | - |
dc.identifier.scopusid | 2-s2.0-84860834238 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.description.scptc | 9 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENTNER-DOUDOROFF PATHWAY | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SULFOLOBUS-SOLFATARICUS | - |
dc.subject.keywordPlus | L-RHAMNULOSE | - |
dc.subject.keywordPlus | GLUCOSE-DEHYDROGENASE | - |
dc.subject.keywordPlus | COENZYME SPECIFICITY | - |
dc.subject.keywordPlus | CARBONYL REDUCTASE | - |
dc.subject.keywordPlus | INTRACELLULAR PH | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ALDOLASE | - |
dc.subject.keywordAuthor | NADP-specific L-rhamnose dehydrogenase | - |
dc.subject.keywordAuthor | Thermoplasma acidophilum | - |
dc.subject.keywordAuthor | Thermophilic enzyme | - |
dc.subject.keywordAuthor | Archaea | - |
dc.subject.keywordAuthor | Non-phosphorylated pathway | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |
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