Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins
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SCOPUS
- Title
- Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins
- Authors
- Jeong, HS; Choi, SM; Kim, HW; Park, JW; Park, HN; Park, SM; Jang, SK; Rhee, YM; Kim, BH
- Date Issued
- 2013-05
- Publisher
- Royal Society of Chemistry
- Abstract
- This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We anticipate that this assay will open up new possibilities for meaningful studies of RNA-RBP interactions.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/15327
- DOI
- 10.1039/C2MB25470K
- ISSN
- 1742-206X
- Article Type
- Article
- Citation
- Molecular BioSystems, vol. 09, no. 5, page. 948 - 951, 2013-05
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