Controlled release of human growth hormone fused with a human hybrid Fc fragment through a nanoporous polymer membrane
SCIE
SCOPUS
- Title
- Controlled release of human growth hormone fused with a human hybrid Fc fragment through a nanoporous polymer membrane
- Authors
- Kim, ES; Jang, DS; Yang, SY; Lee, MN; Jin, KS; Cha, HJ; Kim, JK; SUNG, YOUNG CHUL; CHOI, KWAN YONG
- Date Issued
- 2013-05
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- Nanotechnology has been applied to the development of more effective and compatible drug delivery systems for therapeutic proteins. Human growth hormone (hGH) was fused with a hybrid Fc fragment containing partial Fc domains of human IgD and IgG(4) to produce a long-acting fusion protein. The fusion protein, hGH-hyFc, resulted in the increase of the hydrodynamic diameter (ca. 11 nm) compared with the diameter (ca. 5 nm) of the recombinant hGH. A diblock copolymer membrane with nanopores (average diameter of 14.3 nm) exhibited a constant release rate of hGH-hyFc. The hGH-hyFc protein released in a controlled manner for one month was found to trigger the phosphorylation of Janus kinase 2 (JAK2) in human B lymphocyte and to exhibit an almost identical circular dichroism spectrum to that of the original hGH-hyFc, suggesting that the released fusion protein should maintain the functional and structural integrity of hGH. Thus, the nanoporous release device could be a potential delivery system for the long-term controlled release of therapeutic proteins fused with the hybrid Fc fragment.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/15470
- DOI
- 10.1039/C3NR00474K
- ISSN
- 2040-3364
- Article Type
- Article
- Citation
- NANOSCALE, vol. 10, no. 5, page. 4262 - 4269, 2013-05
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