Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4+ T Lymphocytes
SCIE
SCOPUS
- Title
- Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4+ T Lymphocytes
- Authors
- Jun, SH; Kim, K; An, HJ; Kim, BC; Sonn, CH; Kim, M; Doh, J; Yee, C; Lee, KM; Kim, J
- Date Issued
- 2012-11-07
- Publisher
- wiley
- Abstract
- A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4(+) T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4(+) T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4(+) T cells for the purpose of cell therapies.
- Keywords
- electrospun polymer nanofibers; ethanol dispersion; CD4+T lymphocyte; cell separation; cell transplantation; BIOMEDICAL APPLICATIONS; METASTATIC MELANOMA; PROTEIN DIGESTION; PANCREATIC-CANCER; ELECTROSPUN; DELIVERY; SCAFFOLDS; COATINGS; HER2/NEU
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/15646
- DOI
- 10.1002/ADFM.201200657
- ISSN
- 1616-301X
- Article Type
- Article
- Citation
- ADVANCED FUNCTIONAL MATERIALS, vol. 22, no. 21, page. 4448 - 4455, 2012-11-07
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