DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, J | - |
dc.contributor.author | Seo, SW | - |
dc.contributor.author | Jang, S | - |
dc.contributor.author | Shin, SI | - |
dc.contributor.author | Lim, CH | - |
dc.contributor.author | Roh, TY | - |
dc.contributor.author | Jung, GY | - |
dc.date.accessioned | 2016-03-31T08:47:03Z | - |
dc.date.available | 2016-03-31T08:47:03Z | - |
dc.date.created | 2013-02-27 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.other | 2013-OAK-0000026557 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/16040 | - |
dc.description.abstract | An extension of directed evolution strategies to genome-wide variations increases the chance of obtaining metabolite-overproducing microbes. However, a general high-throughput screening platform for selecting improved strains remains out of reach. Here, to expedite the evolution of metabolite-producing microbes, we utilize synthetic RNA devices comprising a riboswitch and a selection module that specifically sense inconspicuous metabolites. Using L-lysine-producing Escherichia coli as a model system, we demonstrated that this RNA device could enrich pathway-optimized strains to up to 75% of the total population after four rounds of enrichment cycles. Furthermore, the potential applicability of this device was examined by successfully extending its application to the case of L-tryptophan. When used in conjunction with combinatorial mutagenesis for metabolite overproduction, our synthetic RNA device should facilitate strain improvement. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.title | Synthetic RNA devices to expedite the evolution of metabolite-producing microbes | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1038/NCOMMS2404 | - |
dc.author.google | Yang, J | - |
dc.author.google | Seo, SW | - |
dc.author.google | Jang, S | - |
dc.author.google | Shin, SI | - |
dc.author.google | Lim, CH | - |
dc.author.google | Roh, TY | - |
dc.author.google | Jung, GY | - |
dc.relation.volume | 4 | - |
dc.contributor.id | 10130678 | - |
dc.relation.journal | NATURE COMMUNICATIONS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.4 | - |
dc.identifier.wosid | 000316614600083 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 4 | - |
dc.contributor.affiliatedAuthor | Roh, TY | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-84879164975 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 75 | - |
dc.description.scptc | 72 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GENOME-SCALE | - |
dc.subject.keywordPlus | L-TRYPTOPHAN | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | LYSINE | - |
dc.subject.keywordPlus | RIBOSWITCHES | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | SWITCHES | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.