DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gil, JE | - |
dc.contributor.author | Kim, E | - |
dc.contributor.author | Kim, IS | - |
dc.contributor.author | Ku, B | - |
dc.contributor.author | Park, WS | - |
dc.contributor.author | Oh, BH | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Cho, W | - |
dc.contributor.author | Heo, WD | - |
dc.date.accessioned | 2016-03-31T08:51:33Z | - |
dc.date.available | 2016-03-31T08:51:33Z | - |
dc.date.created | 2013-01-31 | - |
dc.date.issued | 2012-11-30 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2012-OAK-0000026233 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/16189 | - |
dc.description.abstract | Protrudin is a FYVE (Fab 1, YOTB, Vac 1, and EEA1) domain-containing protein involved in transport of neuronal cargoes and implicated in the onset of hereditary spastic paraplegia. Our image-based screening of the lipid binding domain library revealed novel plasma membrane localization of the FYVE domain of protrudin unlike canonical FYVE domains that are localized to early endosomes. The membrane binding study by surface plasmon resonance analysis showed that this FYVE domain preferentially binds phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P-2), phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P-2), and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P-3) unlike canonical FYVE domains that specifically bind phosphatidylinositol 3-phosphate (PtdIns(3) P). Furthermore, we found that these phosphoinositides (PtdInsP) differentially regulate shuttling of protrudin between endosomes and plasma membrane via its FYVE domain. Protrudin mutants with reduced PtdInsP-binding affinity failed to promote neurite outgrowth in primary cultured hippocampal neurons. These results suggest that novel PtdInsP selectivity of the protrudin-FYVE domain is critical for its cellular localization and its role in neurite outgrowth. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.title | Phosphoinositides Differentially Regulate Protrudin Localization through the FYVE Domain | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1074/jbc.M112.419127 | - |
dc.author.google | Gil, JE | - |
dc.author.google | Kim, E | - |
dc.author.google | Kim, IS | - |
dc.author.google | Ku, B | - |
dc.author.google | Park, WS | - |
dc.author.google | Oh, BH | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Cho, | - |
dc.relation.volume | 287 | - |
dc.relation.issue | 49 | - |
dc.relation.startpage | 41268 | - |
dc.relation.lastpage | 41276 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.287, no.49, pp.41268 - 41276 | - |
dc.identifier.wosid | 000311887600041 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 41276 | - |
dc.citation.number | 49 | - |
dc.citation.startPage | 41268 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 287 | - |
dc.contributor.affiliatedAuthor | Kim, E | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-84870326195 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 12 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-PHOSPHATE | - |
dc.subject.keywordPlus | MEMBRANE TRAFFICKING | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | EARLY ENDOSOME | - |
dc.subject.keywordPlus | LIVING CELLS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | 4,5-BISPHOSPHATE | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.