Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium
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SCOPUS
- Title
- Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium
- Authors
- Hwang, KY; Cho, CS; Kim, SS; Baek, K; Kim, SH; Yu, YG; Cho, YJ
- Date Issued
- 1999-04
- Publisher
- crystallization papers
- Abstract
- Glutamate racemase catalyzes the reversible reaction of L-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6(1)22 or P6(5)22 with unit-cell parameters a = b = 72.1, c = 185.02 Angstrom. The asymmetric unit contains one molecule, corresponding to a V-m value of 2.35 Angstrom(3) Da(-1). Complete data sets from a native and a mercury-derivative crystal have been collected at 2.0 and 2.3 Angstrom resolution, respectively, using a synchrotron-radiation source.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16312
- DOI
- 10.1107/S0907444999000608
- ISSN
- 2059-7983
- Article Type
- Article
- Citation
- Acta Crystallographica Section D: Biological Crystallography, vol. 55, no. 4, page. 927 - 928, 1999-04
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- There are no files associated with this item.
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