An accurate multiplex antibiotic susceptibility test using a high-resolution CE-SSCP-based stuffer-free multiplex ligation-dependent probe amplification system
SCIE
SCOPUS
- Title
- An accurate multiplex antibiotic susceptibility test using a high-resolution CE-SSCP-based stuffer-free multiplex ligation-dependent probe amplification system
- Authors
- Chung, B; Shin, GW; Choi, W; Hwang, HS; Oh, MH; Jung, GY
- Date Issued
- 2013-01
- Publisher
- WILEY-BLACKWELL
- Abstract
- The success of antimicrobial therapy depends on effective prescription of antibiotics. Assessment of clinical isolates using rapid antimicrobial susceptibility tests allows effective microbiological therapy to be commenced in a timely manner. However, conventional antimicrobial susceptibility testing is time-consuming and laborious. In the present study, we employed stuffer-free multiplex ligation-dependent probe amplification (MLPA) coupled with analysis of single-strand conformation polymorphisms, via high-resolution CE, to develop a multiplex antibiotic susceptibility test. Using this method, parallel analysis of specific genetic markers was employed to determine minimal inhibitory concentration values. The values derived using the stuffer-free MLPA method agreed with those estimated using a conventional broth dilution method. These findings indicate that the stuffer-free MLPA-based approach is a viable alternative to the conventional method.
- Keywords
- Antibiotic susceptibility test; High-resolution CE-SSCP; Multiplex detection; Stuffer-free multiplex ligation-dependent probe amplification; REAL-TIME PCR; CAPILLARY-ELECTROPHORESIS; INFECTIONS; ASSAY; QUANTIFICATION; BACTEREMIA; COMMUNITY; THERAPY; DNA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16345
- DOI
- 10.1002/ELPS.201200372
- ISSN
- 0173-0835
- Article Type
- Article
- Citation
- Electrophoresis, vol. 34, no. 2, page. 284 - 288, 2013-01
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- There are no files associated with this item.
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