DC Field | Value | Language |
---|---|---|
dc.contributor.author | Na Eun Cheong | - |
dc.contributor.author | Yeon Ok Choi | - |
dc.contributor.author | Woe Yeon Kim | - |
dc.contributor.author | In Suck Bae | - |
dc.contributor.author | Moo Je Cho | - |
dc.contributor.author | Hwang, I | - |
dc.contributor.author | Jae Won Kim | - |
dc.contributor.author | Sang Yeol Lee | - |
dc.date.accessioned | 2016-03-31T09:00:42Z | - |
dc.date.available | 2016-03-31T09:00:42Z | - |
dc.date.created | 2012-05-22 | - |
dc.date.issued | 1997-04-30 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 1997-OAK-0000025529 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/16471 | - |
dc.description.abstract | A 28-kDa antifungal PR-5 protein (PLTP) was purified from pumpkin leaves to homogeneity by using ammonium sulfate fractionation, a regenerated chitin column, and reversed-phase column chromatographies on butyl-Toyopearl and HPLC C-18 columns. Analysis of 14 N-terminal amino acid sequences of PLTP shows 100% sequence identity to those of two PR-5 proteins, NP24 from tomatoes and AP24 from tobacco. The identical sequence also exhibited high amino acid sequence homology to that of an osmotin-like protein (OLP; 71%) from tobacco cells and thaumatin (64%), a sweet-tasting protein of Thaumatococcus danielli Bench. When the PLTP was immune-blotted with antiserum raised against the tobacco OLP, the OLP antibody specifically cross-reacted with the PLTP, suggesting that they share several common epitopes in their tertiary structure of the proteins. The purified PLTP rapidly lyzed hyphal tips of Neurospora crassa at a concentration greater than 200 nM and significantly inhibited the fungal growth of Fusarium oxysporum in an agar-disc plate at a concentration greater than 2 mu M. It also shows a synergistic effect with nikkomycin, a chitin synthase inhibitor, for the growth inhibition of Candida albicans. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Korean Society for Molecular and Cellular Biology | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | THAUMATIN-LIKE PROTEINS | - |
dc.subject | TRANSGENIC PLANTS | - |
dc.subject | CDNA | - |
dc.subject | IDENTIFICATION | - |
dc.subject | SEQUENCE | - |
dc.subject | OSMOTIN | - |
dc.subject | MAIZE | - |
dc.subject | CELLS | - |
dc.title | Purification and Characterization of an Antifungal PR-5 Protein from Pumpkin Leaves | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.author.google | Cheong, NE | - |
dc.author.google | Choi, YO | - |
dc.author.google | Kim, WY | - |
dc.author.google | Bae, IS | - |
dc.author.google | Cho, MJ | - |
dc.author.google | Hwang, I | - |
dc.author.google | Kim, JW | - |
dc.author.google | Lee, SY | - |
dc.relation.volume | 7 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 214 | - |
dc.relation.lastpage | 219 | - |
dc.contributor.id | 10078446 | - |
dc.relation.journal | MOLECULES AND CELLS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.7, no.2, pp.214 - 219 | - |
dc.identifier.wosid | A1997WX19900011 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 219 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 214 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 7 | - |
dc.contributor.affiliatedAuthor | Hwang, I | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 25 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | THAUMATIN-LIKE PROTEINS | - |
dc.subject.keywordPlus | TRANSGENIC PLANTS | - |
dc.subject.keywordPlus | CDNA | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | OSMOTIN | - |
dc.subject.keywordPlus | MAIZE | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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