DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, KS | - |
dc.contributor.author | Seung-Jae Lee | - |
dc.contributor.author | Haksue Lee | - |
dc.contributor.author | Moon, W | - |
dc.contributor.author | Cho, DW | - |
dc.date.accessioned | 2016-03-31T09:34:45Z | - |
dc.date.available | 2016-03-31T09:34:45Z | - |
dc.date.created | 2011-08-11 | - |
dc.date.issued | 2011-06-30 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.other | 2011-OAK-0000023763 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17358 | - |
dc.description.abstract | We observed how combined mechanical stimuli affect the proliferation and differentiation of pre-osteoblasts. For this research, a bioreactor system was developed that can simultaneously stimulate cells with cyclic strain and ultrasound, each of which is known to effectively stimulate bone tissue regeneration. MC3T3-E1 pre-osteoblasts were chosen for bone tissue engineering due to their osteoblast-like characteristics. 3-D scaffolds were fabricated with polycaprolactone and poly-L-lactic acid using the salt leaching method. The cells were stimulated by the bioreactor with cyclic strain and ultrasound. The bioreactor was set at a frequency of 1.0 Hz and 10 % strain for cyclic strain and 1.0 MHz and 30 mW/cm(2) for ultrasound. Three experimental groups (ultrasound, cyclic strain, and combined stimulation) and a control group were examined. Each group was stimulated for 20 min/day. Mechanical stimuli did not affect MC3T3-E1 cell proliferation significantly up to 10 days when measured with the cell counting kit-8. However, gene expression analysis of collagen type-I, osteocalcin, RUNX2, and osterix revealed that the combined mechanical stimulation accelerated the matrix maturation of MC3T3-E1 cells. These results indicate that the combined mechanical stimulation can enhance the differentiation of pre-osteoblasts more efficiently than simple stimuli, in spite of no effect on cell proliferation. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.title | Effects of combined mechanical stimulation on the proliferation and differentiation of pre-osteoblasts | - |
dc.type | Article | - |
dc.contributor.college | 기계공학과 | - |
dc.identifier.doi | 10.3858/EMM.2011.43.6.040 | - |
dc.author.google | Kang, KS | - |
dc.author.google | Lee, SJ | - |
dc.author.google | Lee, H | - |
dc.author.google | Moon, W | - |
dc.author.google | Cho, DW | - |
dc.relation.volume | 43 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 367 | - |
dc.relation.lastpage | 373 | - |
dc.contributor.id | 10106244 | - |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.43, no.6, pp.367 - 373 | - |
dc.identifier.wosid | 000292232600006 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 373 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 367 | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 43 | - |
dc.contributor.affiliatedAuthor | Moon, W | - |
dc.contributor.affiliatedAuthor | Cho, DW | - |
dc.identifier.scopusid | 2-s2.0-79959937513 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 29 | - |
dc.description.scptc | 30 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INTENSITY PULSED ULTRASOUND | - |
dc.subject.keywordPlus | MARROW STROMAL CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | MATRIX | - |
dc.subject.keywordPlus | MICROSTEREOLITHOGRAPHY | - |
dc.subject.keywordPlus | CARTILAGE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | bioreactors | - |
dc.subject.keywordAuthor | cell differentiation | - |
dc.subject.keywordAuthor | physical stimulation | - |
dc.subject.keywordAuthor | tissue engineering | - |
dc.subject.keywordAuthor | ultrasound | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.