DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YK | - |
dc.contributor.author | Kim, KR | - |
dc.contributor.author | Kang, DG | - |
dc.contributor.author | Jang, SY | - |
dc.contributor.author | Kim, YH | - |
dc.contributor.author | Cha, HJ | - |
dc.date.accessioned | 2016-03-31T09:35:56Z | - |
dc.date.available | 2016-03-31T09:35:56Z | - |
dc.date.created | 2011-06-16 | - |
dc.date.issued | 2011-05-20 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.other | 2011-OAK-0000023715 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17380 | - |
dc.description.abstract | Previously, we have shown that simple paucimannosidic N-glycan structures in insect Drosophila S2 cells arise mainly because of beta-N-acetylglucosaminidase (GlcNAcase) action. Thus, in an earlier report, we suppressed GlcNAcase activity and clearly demonstrated that more complexN-glycans with two terminal N-acetylglucosamine (GlcNAc) residues were then synthesized. In the present work, we investigated the synergistic effects of beta-1,4-galactosyltransferase (GalT) expression and GlcNAcase suppression on N-glycan patterns. We found that the N-glycan pattern of human erythropoietin secreted by engineered S2 cells expressing GalT but not GlcNAcase was complete, even in small portion, except for sialylation; the N-glycan structures had two terminal galactose (Gal) residues. When GalT was expressed but GlcNAcase was not inhibited, N-glycan with GlcNAc and Gal at only one branch end was synthesized. Therefore, it will be possible to express a complete functional human glycoprotein in engineered Drosophila S2 cells by suppressing GlcNAcase and co-expressing additional glycosyltransferases of N-glycosylation pathway. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF BIOTECHNOLOGY | - |
dc.subject | Drosophila S2 cells | - |
dc.subject | N-Glycosylation | - |
dc.subject | beta-N-Acetylglucosaminidase | - |
dc.subject | beta 1,4-Galactosyltransferase | - |
dc.subject | Human erythropoietin | - |
dc.subject | SECRETED HUMAN ERYTHROPOIETIN | - |
dc.subject | STATISTICAL OPTIMIZATION | - |
dc.subject | GLYCOSYLATION PATHWAY | - |
dc.subject | PROTEIN | - |
dc.subject | GLYCOPROTEIN | - |
dc.subject | MEMBRANE | - |
dc.title | Expression of beta-1,4-galactosyltransferase and suppression of beta-N-acetylglucosaminidase to aid synthesis of complex N-glycans in insect Drosophila S2 cells | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1016/J.JBIOTEC.2011.03.021 | - |
dc.author.google | Kim, YK | - |
dc.author.google | Kim, KR | - |
dc.author.google | Kang, DG | - |
dc.author.google | Jang, SY | - |
dc.author.google | Kim, YH | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 153 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 145 | - |
dc.relation.lastpage | 152 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | JOURNAL OF BIOTECHNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOTECHNOLOGY, v.153, no.3, pp.145 - 152 | - |
dc.identifier.wosid | 000290513700010 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 152 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 145 | - |
dc.citation.title | JOURNAL OF BIOTECHNOLOGY | - |
dc.citation.volume | 153 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-79955773528 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 14 | - |
dc.description.scptc | 14 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SECRETED HUMAN ERYTHROPOIETIN | - |
dc.subject.keywordPlus | STATISTICAL OPTIMIZATION | - |
dc.subject.keywordPlus | GLYCOSYLATION PATHWAY | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GLYCOPROTEIN | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordAuthor | Drosophila S2 cells | - |
dc.subject.keywordAuthor | N-Glycosylation | - |
dc.subject.keywordAuthor | beta-N-Acetylglucosaminidase | - |
dc.subject.keywordAuthor | beta 1,4-Galactosyltransferase | - |
dc.subject.keywordAuthor | Human erythropoietin | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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