Nanoband electrode for high-performance in-channel amperometric detection in dual-channel microchip capillary electrophoresis
SCIE
SCOPUS
- Title
- Nanoband electrode for high-performance in-channel amperometric detection in dual-channel microchip capillary electrophoresis
- Authors
- Chen, C; Teng, W; Hahn, JH
- Date Issued
- 2011-04
- Publisher
- WILEY-BLACKWELL
- Abstract
- A nanoband electrode detector integrated with a dual-channel polydimethylsiloxane microchip is proposed for in-channel amperometric detection in microchip capillary electrophoresis. Gold nanoband electrodes, which were fabricated on SU-8 substrates with a 100-nm-width gold layer, were introduced into the dual-channel microchip to be an electrochemical detector. Due to the nano-sized width of the detector, the noise of the amperometric detection was significantly reduced, and a high separation resolution was achieved for monitoring the analytes. The detection sensitivity of the system was improved by high signal-to-noise ratio, and a low detection limit on microchip was obtained for p-aminophenol (2.09 nM). Because of the high resolution in measuring half-peak width, the plate number that is used to evaluate the separation efficiency was 1.5-fold higher than that using 50-mu m-width electrochemical detector. The effect of sample injection time and data acquisition time on separation efficiency was investigated, and an attractive separation efficiency was achieved with a plate number up to 17 500.
- Keywords
- Capillary electrophoresis; Dual-channel; Electrochemical detection; In-channel detection; Microchip; BAND ELECTRODES; FARADAIC ELECTROCHEMISTRY; MICROELECTRODE ARRAYS; NANOELECTRODE ARRAYS; FABRICATION; ULTRAMICROELECTRODES; POLY(DIMETHYLSILOXANE); MICROCYLINDER; VOLTAMMETRY; DIMENSIONS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17462
- DOI
- 10.1002/ELPS.201000661
- ISSN
- 0173-0835
- Article Type
- Article
- Citation
- ELECTROPHORESIS, vol. 32, no. 8, page. 838 - 843, 2011-04
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