DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bhuiyan, MSU | - |
dc.contributor.author | Min, SR | - |
dc.contributor.author | Jeong, WJ | - |
dc.contributor.author | Sultana, S | - |
dc.contributor.author | Choi, KS | - |
dc.contributor.author | Lim, YP | - |
dc.contributor.author | Song, WY | - |
dc.contributor.author | Lee, Y | - |
dc.contributor.author | Liu, JR | - |
dc.date.accessioned | 2016-03-31T09:43:03Z | - |
dc.date.available | 2016-03-31T09:43:03Z | - |
dc.date.created | 2011-05-16 | - |
dc.date.issued | 2011-01 | - |
dc.identifier.issn | 1342-4580 | - |
dc.identifier.other | 2011-OAK-0000023492 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17503 | - |
dc.description.abstract | An efficient Agrobacterium-mediated genetic transformation method was established for Brassica juncea by investigating several factors responsible for successful gene transfer. Four-day-old cotyledon explants from in vitro grown seedlings were co-cultivated with Agrobacterium strain GV3101 harboring the binary vector EnPCAMBIA1302-YCF1, which contained the hygromycin phosphotransferase (HPT) gene as a selectable marker and the yeast cadmium factor 1 (YCF1) gene. Two days co-cultivation period on shoot induction medium (MS medium supplemented with 0.1 mg l(-1) alpha-naphthaleneacetic acid, 1.0 mg l(-1) 6-benzyladenine, and 2.0 mg l(-1) silver nitrate) containing 20 mg l(-1) acetosyringone and five days delaying exposure of explants to selective agent enhanced transformation efficiency significantly. A three-step selection strategy was developed to select hygromycin resistant shoots. Hygromycin-resistant shoots were subsequently rooted on root induction medium. Rooted plantlets were transferred to pot-soil, hardened, and grown in a greenhouse until maturity. Using the optimized transformation procedure, transformation efficiency reached at 16.2% in this study. Southern blot analysis was performed to confirm that transgenes (HPT and YCF1) were stably integrated into the plant genome. All transgenic plants showed single-copy of transgene integration in the host genome. Segregation analysis of T-1 progeny showed that the transgenes were stably integrated and transmitted to the progeny in a Mendelian fashion. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | JAPANESE SOC PLANT CELL & MOLECULAR BIOL | - |
dc.relation.isPartOf | PLANT BIOTECHNOLOGY | - |
dc.subject | Cotyledon explants | - |
dc.subject | co-cultivation | - |
dc.subject | genetic transformation | - |
dc.subject | hygromycin | - |
dc.subject | Indian mustard | - |
dc.subject | INDIAN MUSTARD | - |
dc.subject | SHOOT REGENERATION | - |
dc.subject | TRANSGENIC PLANTS | - |
dc.subject | TOLERANCE | - |
dc.subject | L. | - |
dc.subject | TUMEFACIENS | - |
dc.subject | LEAF | - |
dc.subject | ACCUMULATION | - |
dc.subject | ANTIBIOTICS | - |
dc.subject | CADMIUM | - |
dc.title | An improved method for Agrobacterium-mediated genetic transformation from cotyledon explants of Brassica juncea | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.5511/PLANTBIOTECHNOLOGY.10.0921A | - |
dc.author.google | Bhuiyan, MSU | - |
dc.author.google | Min, SR | - |
dc.author.google | Jeong, WJ | - |
dc.author.google | Sultana, S | - |
dc.author.google | Choi, KS | - |
dc.author.google | Lim, YP | - |
dc.author.google | Song, WY | - |
dc.author.google | Lee, Y | - |
dc.author.google | Liu, JR | - |
dc.relation.volume | 28 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 17 | - |
dc.relation.lastpage | 23 | - |
dc.contributor.id | 10175128 | - |
dc.relation.journal | PLANT BIOTECHNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCIE | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLANT BIOTECHNOLOGY, v.28, no.1, pp.17 - 23 | - |
dc.identifier.wosid | 000289846900003 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 23 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 17 | - |
dc.citation.title | PLANT BIOTECHNOLOGY | - |
dc.citation.volume | 28 | - |
dc.contributor.affiliatedAuthor | Lee, Y | - |
dc.identifier.scopusid | 2-s2.0-79955148814 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 9 | - |
dc.description.scptc | 12 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INDIAN MUSTARD | - |
dc.subject.keywordPlus | SHOOT REGENERATION | - |
dc.subject.keywordPlus | L. | - |
dc.subject.keywordPlus | TOLERANCE | - |
dc.subject.keywordPlus | LEAF | - |
dc.subject.keywordPlus | ACCUMULATION | - |
dc.subject.keywordPlus | ANTIBIOTICS | - |
dc.subject.keywordPlus | CADMIUM | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | Cotyledon explants | - |
dc.subject.keywordAuthor | co-cultivation | - |
dc.subject.keywordAuthor | genetic transformation | - |
dc.subject.keywordAuthor | hygromycin | - |
dc.subject.keywordAuthor | Indian mustard | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
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