Identification of cellular proteins enhancing activities of internal ribosomal entry sites by competition with oligodeoxynucleotides
SCIE
SCOPUS
- Title
- Identification of cellular proteins enhancing activities of internal ribosomal entry sites by competition with oligodeoxynucleotides
- Authors
- Choi, K; Kim, JH; Li, XY; Paek, KY; Ha, SH; Ryu, SH; Wimmer, E; Jang, SK
- Date Issued
- 2004-02
- Publisher
- OXFORD UNIV PRESS
- Abstract
- The translation of numerous eukaryotic mRNAs is mediated by internal ribosomal entry sites (IRESs). IRES-dependent translation requires both canonical translation initiation factors and IRES-specific trans-acting factors (ITAFs). Here we report a strategy to identify and characterize ITAFs required for IRES-dependent translation. This process involves steps for identifying oligodeoxynucleotides affecting IRES-dependent translation, purifying proteins interacting with the inhibitory DNA, identifying the specific proteins with matrix-assisted laser desorption ionization/time-of-flight mass spectrometry, and confirming the roles of these proteins in IRES-dependent translation by depletion and repletion of proteins from an in vitro translation system. Using this strategy, we show that poly(rC)-binding proteins 1 and 2 enhance translation through polioviral and rhinoviral IRES elements.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18029
- DOI
- 10.1093/NAR/GKH300
- ISSN
- 0305-1048
- Article Type
- Article
- Citation
- NUCLEIC ACIDS RESEARCH, vol. 32, no. 4, page. 1308 - 1317, 2004-02
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