DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rong, R | - |
dc.contributor.author | Ahn, JY | - |
dc.contributor.author | Chen, P | - |
dc.contributor.author | Suh, PG | - |
dc.contributor.author | Ye, KQ | - |
dc.date.accessioned | 2016-03-31T12:40:39Z | - |
dc.date.available | 2016-03-31T12:40:39Z | - |
dc.date.created | 2009-08-12 | - |
dc.date.issued | 2003-12-26 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2004-OAK-0000003899 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/18187 | - |
dc.description.abstract | Phospholipase C-gamma1( PLC-gamma1) hydrolyzes phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate and diacylglycerol (DAG). PLC-gamma1 is implicated in a variety of cellular signalings and processes including mitogenesis and calcium entry. However, numerous studies demonstrate that the lipase activity is not required for PLC-gamma1 to mediate these events. Here, we report that the phospholipase activity of PLC-gamma1 plays an essential role in nerve growth factor (NGF)-triggered Raf/MEK/MAPK pathway activation in PC12 cells. Employing PC12 cells stably transfected with an inducible form of wild-type PLC-gamma1 or lipase inactive PLC-gamma1 with histidine 335 mutated into glutamine in the catalytic domain, we show that NGF provokes robust activation of MAP kinase in wild-type but not in lipase inactive cells. Both Ras/C-Raf/MEK1 and Rap1/B-Raf/MEK1 pathways are intact in the wild-type cells. By contrast, these signaling cascades are diminished in the mutant cells. Pretreatment with cell permeable DAG analog 1-oleyl-2-acetylglycerol rescues the MAP kinase pathway activation in the mutant cells. These observations indicate that the lipase activity of PLC-gamma1 mediates NGF-regulated MAPK signaling upstream of Ras/Rap1 activation probably through second messenger DAG-activated Ras and Rap-GEFs. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | NUCLEOTIDE EXCHANGE FACTOR | - |
dc.subject | SH3 DOMAIN | - |
dc.subject | C-GAMMA | - |
dc.subject | B-RAF | - |
dc.subject | MEDIATED ACTIVATION | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | NUCLEAR GTPASE | - |
dc.subject | DNA-SYNTHESIS | - |
dc.subject | C-GAMMA-1 | - |
dc.subject | PLC-GAMMA-1 | - |
dc.title | Phospholipase activity of phospholipase C-gamma 1 is required for nerve growth factor-regulated MAP kinase signaling cascade in PC12 cells | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/JBC.M3067442 | - |
dc.author.google | Rong, R | - |
dc.author.google | Ahn, JY | - |
dc.author.google | Chen, P | - |
dc.author.google | Suh, PG | - |
dc.author.google | Ye, KQ | - |
dc.relation.volume | 278 | - |
dc.relation.issue | 52 | - |
dc.relation.startpage | 52497 | - |
dc.relation.lastpage | 52503 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.278, no.52, pp.52497 - 52503 | - |
dc.identifier.wosid | 000187480700064 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 52503 | - |
dc.citation.number | 52 | - |
dc.citation.startPage | 52497 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 278 | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 18 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NUCLEOTIDE EXCHANGE FACTOR | - |
dc.subject.keywordPlus | SH3 DOMAIN | - |
dc.subject.keywordPlus | C-GAMMA | - |
dc.subject.keywordPlus | B-RAF | - |
dc.subject.keywordPlus | MEDIATED ACTIVATION | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | NUCLEAR GTPASE | - |
dc.subject.keywordPlus | DNA-SYNTHESIS | - |
dc.subject.keywordPlus | C-GAMMA-1 | - |
dc.subject.keywordPlus | PLC-GAMMA-1 | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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