A role for phosphatidylinositol 3-phosphate in abscisic acid-induced reactive oxygen species generation in guard cells
SCIE
SCOPUS
- Title
- A role for phosphatidylinositol 3-phosphate in abscisic acid-induced reactive oxygen species generation in guard cells
- Authors
- Park, KY; Jung, JY; Park, J; Hwang, JU; Kim, YW; Hwang, I; Lee, Y
- Date Issued
- 2003-05
- Publisher
- AMER SOC PLANT BIOLOGISTS
- Abstract
- Guard cells generate reactive oxygen species (ROS) in response to abscisic acid (ABA), which leads to stomatal closing. The upstream steps of the ABA-induced ROS generation pathway remain largely unknown. In animal cells, ROS generation in neutrophils is activated by phosphatidylinositol 3-phosphate (PI3P). Stomatal guard cells contain PI3P and PI 3-kinase activity. In this study, we tested whether PI3P has a role in ROS generation in guard cells exposed to ABA. We found that PI 3-kinase inhibitors wortmannin or LY294002 inhibited ABA-induced ROS generation and stomatal closing. Endosome-binding domain (of human EEA1), which specifically binds to PI3P, also inhibited ABA-induced ROS generation and stomatal closing when overexpressed in guard cells. Hydrogen peroxide partially reversed the effects of wortmannin or LY294002 on ABA-induced stomatal closing. These results support a role for PI3P in ABA-induced ROS generation and stomatal closing movement.
- Keywords
- HYDROGEN-PEROXIDE; CLADOSPORIUM-FULVUM; PLASMA-MEMBRANE; CHANNELS; TOMATO; ELICITORS; OXIDASE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18362
- DOI
- 10.1104/pp.102.016964
- ISSN
- 0032-0889
- Article Type
- Article
- Citation
- PLANT PHYSIOLOGY, vol. 132, no. 1, page. 92 - 98, 2003-05
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