Protein kinase A- and C-induced insulin release from Ca2+-insensitive pools
SCIE
SCOPUS
- Title
- Protein kinase A- and C-induced insulin release from Ca2+-insensitive pools
- Authors
- Lee, IS; Hur, EM; Suh, BC; Kim, MH; Koh, DS; Rhee, IJ; Ha, H; Kim, KT
- Date Issued
- 2003-05
- Publisher
- ELSEVIER SCIENCE INC
- Abstract
- Insulin secretion is known to depend on an increase in intracellular Ca2+ concentration ([Ca2+](i)). However, recent studies have suggested that insulin secretion can also be evoked in a Ca2+-independent manner. In the present study we show that treatment of intact mouse islets and RINm5F cells with protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) or protein kinase A (PKA) activator forskolin promoted insulin secretion with no changes of [Ca2+](i). Moreover, insulin secretion mediated by PMA or forskolin was maintained even when extracellular or cytosolic Ca2+ was deprived by treatment of cells with ethylene glycol bis(beta-amino ethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) or 1,2-bis(2-amino phenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxy methyl ester) (BAPTA/AM) in RINm5F cells. The secretagogue actions of PMA and forskolin were blocked by GF109203X and H89, selective inhibitors for PKC and PKA, respectively. PMA treatment caused translocation of PKC-alpha and PKC-epsilon from cytosol to membrane, implying that selectively PKC-alpha and PKC-epsilon isoforms might be important for insulin secretion. Co-treatment with high K+ and PMA showed a comparable level of insulin secretion to that of PMA alone. In addition, PMA and forskolin evoked insulin secretion in cells where Ca2+-dependent insulin secretion was completed. Our data suggest that PKC and PKA can elicit insulin secretion not only in a Ca2+-sensitive manner but also in a Ca2+-independent manner from separate releasable pools. (C) 2002 Elsevier Science Inc. All rights reserved.
- Keywords
- PKA; PKC; Ca2+-insensitive; insulin; secretion; RINm5F cells; PANCREATIC BETA-CELLS; CYCLIC-AMP; SIGNAL-TRANSDUCTION; CA-2+ CONCENTRATION; INTRACELLULAR CA2+; EXTRACELLULAR CA2+; CYTOSOLIC CA2+; SECRETION; GLUCOSE; EXOCYTOSIS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18605
- DOI
- 10.1016/S0898-6568(02)00137-7
- ISSN
- 0898-6568
- Article Type
- Article
- Citation
- CELLULAR SIGNALLING, vol. 15, no. 5, page. 529 - 537, 2003-05
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.