DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, J | - |
dc.contributor.author | Park, S | - |
dc.contributor.author | Kim, D | - |
dc.contributor.author | Rhim, J | - |
dc.contributor.author | Kim, I | - |
dc.contributor.author | Choi, I | - |
dc.contributor.author | Yi, K | - |
dc.contributor.author | Ryu, S | - |
dc.contributor.author | Suh, P | - |
dc.contributor.author | Chung, D | - |
dc.contributor.author | Bae, Y | - |
dc.contributor.author | Shin, Y | - |
dc.contributor.author | Park, SH | - |
dc.date.accessioned | 2016-03-31T12:57:04Z | - |
dc.date.available | 2016-03-31T12:57:04Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2002-12 | - |
dc.identifier.issn | 0171-5216 | - |
dc.identifier.other | 2003-OAK-0000003085 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/18769 | - |
dc.description.abstract | Purpose: In the present study an antigen-mimetic peptide of the anti-JL1 leukemia-specific monoclonal antibody (mAb) was identified and characterized. Methods: From combinatorial peptide phage display libraries displaying the random linear heptapeptides and dodecapeptides, we selected clones with affinity to anti-JL1 mAb through repeated rounds of panning on a mAb-coated ELISA plate. The antigenicity and immunogenicity of the peptide epitopes were then studied using chemically synthesized peptides. Results: The selected clones had the LXPSIP consensus sequence. Two synthetic peptides LPPSIPFGLTVGGGGS and LLPSIPNQAYLGGGGS specifically reacted with anti-JL1 mAb in ELISA. These two peptides were found to inhibit the interaction between anti-JL1 mAb and JL1 antigen-positive Molt-4 cells. Although the immune sera raised against the keyhole limpet hemocyanin-conjugated peptides failed to react with Molt-4 cells, it showed strong reactivity to the peptide epitope. However, one mAb raised by peptide immunization successfully bound to Molt-4 cells. Conclusion: An epitope-mimetic peptide of anti-JL1 mAb was found using combinatorial peptide phage display libraries. It induced strong humoral response against itself, but only a limited fraction of this humoral response was cross-reactive with the original JL1 antigen. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER-VERLAG | - |
dc.relation.isPartOf | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | - |
dc.subject | phage display | - |
dc.subject | combinatorial peptide library | - |
dc.subject | mimotope-monoclonal antibody | - |
dc.subject | JL1 antigen | - |
dc.subject | DISCONTINUOUS EPITOPES | - |
dc.subject | JL1 | - |
dc.subject | MIMOTOPES | - |
dc.subject | IMMUNIZATION | - |
dc.subject | GENERATION | - |
dc.subject | INHIBITION | - |
dc.subject | EXPRESSION | - |
dc.subject | THYMOCYTE | - |
dc.subject | MIMICKING | - |
dc.subject | MOLECULE | - |
dc.title | Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1007/s00432-002-0390-x | - |
dc.author.google | Chung, J | - |
dc.author.google | Park, S | - |
dc.author.google | Kim, D | - |
dc.author.google | Rhim, J | - |
dc.author.google | Kim, I | - |
dc.author.google | Choi, I | - |
dc.author.google | Yi, K | - |
dc.author.google | Ryu, S | - |
dc.author.google | Suh, P | - |
dc.author.google | Chung, D | - |
dc.author.google | Bae, Y | - |
dc.author.google | Shin, Y | - |
dc.author.google | Park, SH | - |
dc.relation.volume | 128 | - |
dc.relation.issue | 12 | - |
dc.relation.startpage | 641 | - |
dc.relation.lastpage | 649 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, v.128, no.12, pp.641 - 649 | - |
dc.identifier.wosid | 000180042500002 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 649 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 641 | - |
dc.citation.title | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | - |
dc.citation.volume | 128 | - |
dc.contributor.affiliatedAuthor | Ryu, S | - |
dc.contributor.affiliatedAuthor | Suh, P | - |
dc.identifier.scopusid | 2-s2.0-0036454119 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 10 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | DISCONTINUOUS EPITOPES | - |
dc.subject.keywordPlus | JL1 | - |
dc.subject.keywordPlus | MIMOTOPES | - |
dc.subject.keywordPlus | IMMUNIZATION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | THYMOCYTE | - |
dc.subject.keywordPlus | MIMICKING | - |
dc.subject.keywordPlus | MOLECULE | - |
dc.subject.keywordAuthor | phage display | - |
dc.subject.keywordAuthor | combinatorial peptide library | - |
dc.subject.keywordAuthor | mimotope-monoclonal antibody | - |
dc.subject.keywordAuthor | JL1 antigen | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
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