DC Field | Value | Language |
---|---|---|
dc.contributor.author | Goh, CH | - |
dc.contributor.author | Dietrich, P | - |
dc.contributor.author | Steinmeyer, R | - |
dc.contributor.author | Schreiber, U | - |
dc.contributor.author | Nam, HG | - |
dc.contributor.author | Hedrich, R | - |
dc.date.accessioned | 2016-03-31T12:58:07Z | - |
dc.date.available | 2016-03-31T12:58:07Z | - |
dc.date.created | 2009-03-20 | - |
dc.date.issued | 2002-11 | - |
dc.identifier.issn | 0960-7412 | - |
dc.identifier.other | 2002-OAK-0000003030 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/18810 | - |
dc.description.abstract | Stomata open in response to red and blue light. Red light-induced stomatal movement is mediated by guard cell chloroplasts and related to K+-uptake into these motor cells. We have combined a new type of microchlorophyll fluorometer with the patch-clamp technique for parallel studies of the photosynthetic electron transport and activity of plasma membrane K+ channels in single guard cell protoplast. In the whole-cell configuration and presence of ATP in the patch-pipette, the activity of the K+-uptake channels remained constant throughout the course of an experiment (up to 30 min) while photosynthetic activity declined to about 50%. In the absence of ATP inward K+ currents declined in a time-dependent manner. Under these ATP-free conditions, photosynthetic electron transport was completely blocked within 8 min. ADP together with orthophosphate was able to prevent inhibition of photosynthetic electron transport and run-down of K+-channel activity. The results demonstrate that the combination of these two techniques is suited to directly study cytosolic factors as common regulators of photosynthesis and plasma membrane transport within a single-cell. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | BLACKWELL PUBLISHING LTD | - |
dc.relation.isPartOf | PLANT JOURNAL | - |
dc.subject | ATP | - |
dc.subject | chlorophyll fluorescence | - |
dc.subject | electron transport | - |
dc.subject | guard cell | - |
dc.subject | K+ channel | - |
dc.subject | patch-clamp | - |
dc.subject | PLASMA-MEMBRANE | - |
dc.subject | VOLTAGE-DEPENDENCE | - |
dc.subject | KAT1 CHANNEL | - |
dc.subject | PROTON PUMP | - |
dc.subject | PLANT-CELL | - |
dc.subject | PROTOPLASTS | - |
dc.subject | LIGHT | - |
dc.subject | MODULATION | - |
dc.subject | HYPERPOLARIZATION | - |
dc.subject | ACIDIFICATION | - |
dc.title | Parallel recordings of photosynthetic electron transport and K+-channel activity in single guard cells | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1046/j.1365-313X.2002.01451.x | - |
dc.author.google | Goh, CH | - |
dc.author.google | Dietrich, P | - |
dc.author.google | Steinmeyer, R | - |
dc.author.google | Schreiber, U | - |
dc.author.google | Nam, HG | - |
dc.author.google | Hedrich, R | - |
dc.relation.volume | 32 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 623 | - |
dc.relation.lastpage | 630 | - |
dc.contributor.id | 10087591 | - |
dc.relation.journal | PLANT JOURNAL | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLANT JOURNAL, v.32, no.4, pp.623 - 630 | - |
dc.identifier.wosid | 000179414100018 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 630 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 623 | - |
dc.citation.title | PLANT JOURNAL | - |
dc.citation.volume | 32 | - |
dc.contributor.affiliatedAuthor | Nam, HG | - |
dc.identifier.scopusid | 2-s2.0-0038646652 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 17 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | VOLTAGE-DEPENDENCE | - |
dc.subject.keywordPlus | KAT1 CHANNEL | - |
dc.subject.keywordPlus | PROTON PUMP | - |
dc.subject.keywordPlus | PROTOPLASTS | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordPlus | HYPERPOLARIZATION | - |
dc.subject.keywordPlus | RESPIRATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | ATP | - |
dc.subject.keywordAuthor | chlorophyll fluorescence | - |
dc.subject.keywordAuthor | electron transport | - |
dc.subject.keywordAuthor | guard cell | - |
dc.subject.keywordAuthor | K+ channel | - |
dc.subject.keywordAuthor | patch-clamp | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
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