DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baek, KH | - |
dc.contributor.author | Ha, SJ | - |
dc.contributor.author | Sung, YC | - |
dc.date.accessioned | 2016-03-31T13:11:59Z | - |
dc.date.available | 2016-03-31T13:11:59Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2001-09-01 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.other | 2001-OAK-0000002352 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19291 | - |
dc.description.abstract | Phosphorothioate cytosine-guanine oligodeoxynucleotides (CpG PS-ODNs) has been reported to induce Th1 immune responses against coadministered Ags more efficiently than phosphodiester CpG ODNs (CpG PO-ODNs). Here, we demonstrated that PS-ODNs, but not PO-ODNs, have a chemotactic effect on primary macrophages, which is independent of the CpG motif. In addition, the conjugation of a hexameric dG run (dG(6) run) at the 3' terminus reduced the concentration required for the optimal chemotactic activity of PS-ODNs by similar to 10-fold. Endosomal maturation blockers, such as monensin and chloroquine, inhibited the chemotactic effect of PS-ODNs. The inhibition of the activities of p38 mitogen-activated protein (MAP) kinase, and extracellular signal-related kinases (ERKs) as well as phosphoinositide 3-kinase with their specific inhibitors also resulted in suppressing the chemotaxis of primary macrophages induced by PS-ODNs. These results indicate that the PS-ODN-mediated chemotaxis requires the activation of ERKs, p38 MAP kinase, and phosphoinositide 3-kinase as well as endosomal maturation. In addition, the phosphorylations of the p38 MAP kinase, ERKs, and protein kinase B, Akt, were induced by PS-ODN, which were further enhanced by the presence of both a dG(6) run and CpG motifs. Our findings suggest that the chemotactic activity of PS-ODNs may be one of the mechanisms by which PS-ODNs exhibit stronger immunomodulatory activities than PO-ODNs in vivo. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.subject | ACTIVATED PROTEIN-KINASES | - |
dc.subject | ANTIGEN-PRESENTING CELLS | - |
dc.subject | CPG OLIGODEOXYNUCLEOTIDES | - |
dc.subject | BACTERIAL-DNA | - |
dc.subject | IMMUNOSTIMULATORY DNA | - |
dc.subject | SULFUR-DIOXIDE | - |
dc.subject | CUTTING EDGE | - |
dc.subject | B-CELL | - |
dc.subject | IL-12 PRODUCTION | - |
dc.subject | DENDRITIC CELLS | - |
dc.title | A novel function of phosphorothioate oligodeoxynucleotides as chemoattractants for primary macrophages | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.4049/jimmunol.167.5.2847 | - |
dc.author.google | Baek, KH | - |
dc.author.google | Ha, SJ | - |
dc.author.google | Sung, YC | - |
dc.relation.volume | 167 | - |
dc.relation.issue | 5 | - |
dc.relation.startpage | 2847 | - |
dc.relation.lastpage | 2854 | - |
dc.contributor.id | 10053752 | - |
dc.relation.journal | JOURNAL OF IMMUNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, v.167, no.5, pp.2847 - 2854 | - |
dc.identifier.wosid | 000172391800053 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 2854 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 2847 | - |
dc.citation.title | JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 167 | - |
dc.contributor.affiliatedAuthor | Sung, YC | - |
dc.identifier.scopusid | 2-s2.0-0035451664 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 34 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASES | - |
dc.subject.keywordPlus | CPG OLIGODEOXYNUCLEOTIDES | - |
dc.subject.keywordPlus | BACTERIAL-DNA | - |
dc.subject.keywordPlus | IMMUNOSTIMULATORY DNA | - |
dc.subject.keywordPlus | CUTTING EDGE | - |
dc.subject.keywordPlus | IL-12 PRODUCTION | - |
dc.subject.keywordPlus | CELL-ACTIVATION | - |
dc.subject.keywordPlus | SULFUR-DIOXIDE | - |
dc.subject.keywordPlus | MOTIFS | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
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