Enhancement of VP1-specific immune responses and protection against EMCV-K challenge by co-delivery of IL-12 DNA with VP1 DNA vaccine
SCIE
SCOPUS
- Title
- Enhancement of VP1-specific immune responses and protection against EMCV-K challenge by co-delivery of IL-12 DNA with VP1 DNA vaccine
- Authors
- Suh, YS; Ha, SJ; Lee, CH; Sin, JI; Sung, YC
- Date Issued
- 2001-02-28
- Publisher
- ELSEVIER SCI LTD
- Abstract
- It has been reported that co-delivery of IL-12 DNA with a DNA vaccine further enhances antigen (Ag)-specific protective immunity in pathogenic challenge models. However. the enhancing effects of antibody by IL-12 have been controversial. To clarify this issue, we constructed an IL-12 expression vector, co-immunized IL-12 DNA with an encephalomycocarditis virus (EMCV)-D VPI plasmid vaccine, and then evaluated immune modulatory effects and protection against lethal EMCV-K challenge. We observed that VP1-specific IgG production, as well as seroconversion rates, were significantly enhanced by IL-12 co-injection. indicating that IL-12 can enhance antibody responses in this model system. In particular, co-injection with VPI plus IL-12 DNA into the same leg enhanced systemic Ag-specific Ige production to a significantly greater extent than either the separate leg injection of VPI and IL-12 DNA or VPI DNA vaccine alone. This suggests that local co-expression of IL-12 along with antigens is more important for enhanced antibody production. Furthermore, IgG2a isotype was significantly enhanced by IL-12 DNA co-injection. indicating a Th1 bias. In addition, co-delivery of IL-12 DNA was demonstrated to enhance VP1-specific Th cell proliferative responses. When animals were challenged with a lethal dose of EMCV-K, IL-12 DNA-co-immunized animals exhibited enhanced survival, as compared to VP1 DNA vaccine alone. These studies suggest that IL-12 plays an important role in increasing Ag-specific Th1 type antibody and cellular responses, resulting in enhanced protection against lethal EMCV-K challenge. (C) 2001 Elsevier Science Ltd. All rights reserved.
- Keywords
- IL-12; EMCV VP1; protection; Th1/Th2; STIMULATORY FACTOR NKSF; MOUSE SKELETAL-MUSCLE; IN-VIVO TRANSFECTION; ENCEPHALOMYOCARDITIS VIRUS; DENDRITIC CELLS; SURFACE-ANTIGEN; MENGO-VIRUS; FACTOR GENE; D-VARIANT; CYTOKINE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19625
- DOI
- 10.1016/S0264-410X(00)00443-6
- ISSN
- 0264-410X
- Article Type
- Article
- Citation
- VACCINE, vol. 19, no. 15-16, page. 1891 - 1898, 2001-02-28
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