Immunological characterization of 130 kDa phospholipase C-beta 4 isozyme in rat cerebellar Purkinje cells
SCIE
SCOPUS
- Title
- Immunological characterization of 130 kDa phospholipase C-beta 4 isozyme in rat cerebellar Purkinje cells
- Authors
- Min, DS; Kim, MJ; Jeong, HK; Lee, YH; Kim, H; Shin, HS; Ryu, SH; Suh, PG
- Date Issued
- 2000-09-29
- Publisher
- ELSEVIER SCI IRELAND LTD
- Abstract
- We have developed a monoclonal antibody, beta 4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-beta 4 (PLC-beta 4). Tissue distribution of 130 kDa PLC-beta 4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-beta 4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-beta 4 in cerebellum was gradually increased from 5 days after birth to adulthood. in the adult cerebellum, especially high level of 130 kDa PLC-beta 4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-beta 4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
- Keywords
- phospholipase C-beta 4; monoclonal antibody; immunohistochemistry; Purkinje cell; phosphoinositide-specific phospholipase C
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19848
- DOI
- 10.1016/S0304-3940(00)01419-1
- ISSN
- 0304-3940
- Article Type
- Article
- Citation
- NEUROSCIENCE LETTERS, vol. 292, no. 1, page. 9 - 12, 2000-09-29
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- There are no files associated with this item.
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